siRNA沉默STAT5对HeLa细胞增殖和凋亡的影响及分子机制

Influence of STAT5 siRNA on HeLa cell proliferation and apoptosis and its molecular mechanism

目的:研究STAT5对宫颈癌HeLa细胞生物学特性的影响及其分子机制。方法:用MTT法测定STAT5 siRNA对HeLa细胞增殖的影响,流式细胞仪检测STAT5 siRNA对HeLa细胞周期和细胞凋亡的影响,用Western blot和RT-PCR分析STAT5、Bcl-2和p53的变化。结果:STAT5 siRNA能够抑制HeLa细胞的增殖,S和G2/M期肿瘤细胞比例减少,G0/G1期肿瘤细胞比例显著增加(P<0.01),并诱导肿瘤细胞凋亡(P<0.01)。STAT5 siRNA下调STAT5、Bcl-2表达且上调p53表达。结论:STAT5 siRNA可以有效地诱导肿瘤细胞的凋亡,并抑制肿瘤细胞的增殖。

Objective：To study effect of STAT5 siRNA on HeLa cytobiology characteristics.Methods：Effect of STAT5 siRNA on HeLa cell proliferation was determined by MTT.Influence of STAT5 siRNA on HeLa cell life cycle and apoptosis was detected by FACS.Changes of STAT5,Bcl-2 and p53 were analyzed by Western blot and RT-PCR.Results：STAT5 siRNA could restrain HeLa cell proliferation,decrease S and G2/M phase cell ratio and increase G0/G1 phase cell ratio,induce tumor cell apoptosis（P〈0.01）.STAT5 siRNA could decrease STAT5,Bcl-2 and increase expression of p53（P〈0.01）.Conclusion：STAT5 siRNA will induce tumor cell apoptosis and inhibit its proliferation.