摘要
利用相关序列扩增多态性(SRAP)分子标记,以柊树[Osmanthus heterophyllus(G. Don.)P. S. Green]和华东木犀(Osmanthus cooperi Hemsl.)为对照种,研究了桂花(Osmanthus fragrans Lour.)88个品种、1个野生种的亲缘关系,18对SRAP引物共获得296个位点,其中248个为多态性位点,多态性比率达83.78%。平均每对引物组合产生16.4个位点和13.8个多态性位点。其中,银桂品种群的Shan-non信息指数(0.3412)和遗传多样性指数(0.2191)最高。遗传变异估算表明:桂花遗传分化系数为52.95%,大部分变异存在于品种群之间,说明品种群体间遗传分化高。聚类分析结果表明,以遗传相似系数0.762为截值,可将91份种质分成6类;各品种群的品种往往聚在一起;四季桂品种群与其他品种群遗传距离较远;色质较深的金桂往往与丹桂品种群的多个品种聚在一起。基于SRAP分子标记的聚类结果与基于形态的传统分类学的结果基本相符。
Interspecific relationships of 88 cultivars and 1 wild species of Osmanthus fragrans were evaluated by using sequence-related amplified polymorphism（SRAP）marker,with O.heterophyllus（G.Don.）P.S.Green and O.cooperi Hemsl.as contrast species.Eighteen primer pairs produced 296 loci,out of which 248 were polymorphic,the percentage of polymorphic loci was 83.78%.An average of 16.4 loci and 13.8 polymorphic loci were generated by each pair of primers.The Shannon＇s index（0.3412）and genetic diversity（0.2191）within Albus Group were the highest among 4 groups of O.fragrans.A high level of genetic differentiation among O.fragrans groups was detected based on Nei＇s genetic diversity analysis（52.95%）.The cluster analysis using arithmetic averages（UPGMA）showed that six clades existed in O.fragrans groups when genetic similarity coefficient was given as 0.762.The genetic distance between Asiaticus Group and other 3 groups were far.Cultivars with deep color in Luteus Group often clustered together with several cultivars in Aurantiacus Group.Cluster analysis based on SRAP marker is generally consistent with taxonomic result based on morphology.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第11期1667-1675,共9页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30771761)
