生物可降解肋骨骨折内固定材料-蚕丝纤维增强纯聚己内酯的体内降解实验研究

Empirical study of degradation performance of silk-fiber-reinforced polycaprolactone composite in vivo

目的对纯聚己内酯(PCL)和蚕丝纤维增强PCL多孔复合材料进行体内降解实验研究,观察其降解速度变化及组织反应情况,为临床应用于多发性肋骨骨折固定材料提供实验依据。方法将纯PCL和蚕丝纤维增强PCL植入兔背部脊柱两侧皮下和肌肉之间,于2、4、8、12、16、24周取材,分别测试2种材料的生物吸收率、组织学观察(HE染色)和扫描电镜观察。结果蚕丝纤维增强PCL在体内植入过程中生物吸收率大于纯PCL,材料内部结构断裂融合降解明显,未见材料周围组织变性、坏死或异常增生现象。结论蚕丝纤维增强PCL多孔复合材料植入体内后具有合适的降解速度,异物反应小,是具有良好应用价值的新型肋骨骨折内固定材料。

Objective To investigate the degradation performance of pure polycaprolactone （PCL） and silkfiber-reinforced PCL composite in vivo in order to provide scientific basis for clinical application of internal fixation materials for multiple ribs fractures. Methods Pure PCL and silk-fiber-reinforced PCL were implanted in the dorsal muscle of rabbits at 2nd,4th, 8th, 12th, 16th and 24th weeks. Then weight loss measurement, histological and SEM observation were performed to study the change of the tested materials in the process of degradation. Results In the process of degradation of silk-fiber-reinforced PCL, the rate of biological absorption was faster than that of pure PCL. The internal structure of the material was obviously degraded. Hyperplasia, necrosis and other rejection reactions were not found after implanting silk-fiber-reinforced PCL composite. Conclusion The silk-fiber-reinforced PCL composite presents the advantages of suitable degradation speed and little foreign body reaction, and is a prospective biomaterial in clinical use for fixation and repair of fracture of multiple ribs.