兔实验性动脉粥样硬化进程中全血粘弹性改变的机制初探

Mechanisms of changes of whole blood viscoelasticity in atheroscherotic rabbits

目的：了解兔实验性动脉粥样硬化（ＡＳ）进程中全血粘弹性的改变及其可能的机制。方法：高铁－硫酸显色法测定总胆固醇（Ｔｃｈ）浓度。膜蛋白含量测定用Ｌｏｗｒｙ法。膜收缩蛋白（ＳＰ）含量测定用ＳＤＳ－聚丙烯酰胺凝胶电泳法。结果：在ＡＳ进程中Ｔｃｈ浓度及ＡＧ２全血粘弹性（动粘度η’和弹性模量Ｇ’）明显升高，而钠泵活性及ＳＰ相对含量降低。Ｔｃｈ浓度与钠泵活性和ＳＰ含量间、Ｇ’与ＳＰ含量间（仅在较高角频率时）呈负相关关系，而钠泵活性与ＳＰ含量间、Ｇ’与η’间呈正相关关系。结论：Ｔｃｈ浓度的升高与钠泵活性降低间有密切关联，而它们的改变与ＳＰ含量降低有一定关系。

Abstract AIM:To investigate the changes of serum total cholesterol(Tch), erythrocyte membrane sodium pump activity (SPA), spectrin(SP) content, whole blood dynamic viscosity(η′) and dynamic modulus (G′) in the course of rabbit atherosclerosis (AS) METHODS:30 rabbits were given cholesterol 1g/d in attempting to obtain an experimental model of AS RESULTS:Tch concentration, η′ and G′ were elevated, while SPA and SP content of red blood cells decreased in the course of AS Tch concentration was negatively correlated with SPA and SP content, on the contrary, SPA was positively correlated with SP content but G′ was negatively correlated with SP content (only under the circumstances of higher ω) G′ was also positively correlated with η′ CONCLUSIONS: Hypercholesterol (Hch) is associated with the decrease in erythrocyte SPA and SP content Hch and the decrease of SPA and SP content play an important role in the course of AS by changing whole blood viscoelasticity MeSH Atherosclerosis; Cholesterol; Sodium; Spectrin