TNFα、IL-1β及IL-6对心脏微血管内皮细胞与中性粒细胞粘附的影响

The effect of TNFα、IL-1 β and IL-6 on the adherence of neutrophils to cardiac microvascular endothelial cells in rats

目的和方法：应用心脏微血管内皮细胞体外培养模型，研究不同浓度的ＴＮＦα、ＩＬ－１β、ＩＬ－６对微血管内皮与中性粒细胞粘附的调节。结果：５×１０４Ｕ／Ｌ的ＴＮＦα和ＩＬ－１β可明显增加内皮细胞与中性粒细胞的粘附（诱导内皮６ｈ），并分别于１０５Ｕ／Ｌ、２×１０５Ｕ／Ｌ浓度时使中性粒细胞粘附数达高峰，但不同浓度的ＩＬ－６均未使粘附增加，免疫组化ＡＢＣ法证实了同样的结果。进一步用抗ＩＣＡＭ－１和抗ＬＦＡ－１单克隆抗体阻断实验表明，两种抗体在５ｍｇ／Ｌ浓度下可显著阻断中性粒细胞与内皮细胞间的粘附。结论：心脏微血管内皮细胞与中性粒细胞的粘附主要通过粘附分子ＩＣＡＭ－１／ＬＦＡ－１介导。

Abstract AIM and METHOD:The effect of TNFα,IL-1β and IL-6 on the neutrophils-cardiac microvascular endothelial cells adhesion were investigated RESULTS:Adherence of rat neutropils to endothelial cells was significantly stimulated by TNFα(50U/mL)and the same consentration of IL-1β( P <0 05).The effect of TNFα and IL-1β tended to be dose dependent.On the other hand,IL-6 had no significant effect on adhesion of neutropils-endothelium.In the immunohistochemical study,cultured endothelial cells constitutively expressed ICAM-1,and ICAM-1 expression was significantly stimulated by TNFα and IL-1β. Meanwhile,adhesion of neutropils-endothelial cells stimulated by TNFα and IL-1β could be partly blocked by anti ICAM-1 and anti LFA-1 antibodies.The blockage rate was 56 2% and 47.3% respectively.CONCLUSION:Adherence of neutropils to endothelial cells was chiefly mediated by ICAM-1/LFA-1 MeSH Endothelium;Cells;Tumor necrosis factor;Interleukins;Neutrophils