维生素E对冷适应大鼠红细胞膜钠-钾-ATP酶活性及丙二醛含量的影响

Effects of dietary VE on Na +-K +-ATPase activity and MDA content of RBC membrane in cold-adaptation rats

目的：研究膳食维生素Ｅ（ＶＥ）增强冷适应的机理。方法：用ＳＤ大鼠为研究对象，分别饲以高ＶＥ饲料（２３０ｍｇ／ｋｇ．饲料）和低ＶＥ饲料（３０ｍｇ／ｋｇ．饲料）。观察大鼠红细胞膜钠－钾－ＡＴＰ酶（Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ）活性和丙二醛（ＭＤＡ）含量的变化。结果：冷适应大鼠红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性明显高于对照组（Ｐ＜０．０５），高ＶＥ摄入大鼠红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性明显高于低ＶＥ摄入组大鼠（Ｐ＜０．０５）；高ＶＥ摄入组大鼠红细胞膜ＭＤＡ含量明显低于低ＶＥ摄入组大鼠（Ｐ＜０．０５）；膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性和ＭＤＡ含量间呈明显负相关。结论：膳食ＶＥ可以提高冷适应大鼠红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性。

Abstract AIM: To evaluate the mechanisms of dietary Vitamin E (VE) enhancing rats to cold adaptation. METHODS: We investigated the Na +-K +-ATPase activity and MDA content of RBC membrane in both cold-adaptation and control rats, which fed either high dietary VE(HVE, 230mg/kg) or low dietary VE(LVE, 30mg/kg). RESULTS: Na +-K +-ATPase activity in cold adaptation rats' RBC membrane were higher than those of control rats', and high dietary VE could increase the activity of Na +-K +-ATPase of rats' RBC membrane. MDA content in RBC membrane of high VE fed rats were markedly lower than that of low VE fed rats. There is a negative correlation between Na +-K +-ATPase activities and MDA contents in RBC membrane. CONCLUSION: Dietary VE could increase Na +-K +-ATPase activity and decrease MDA content in rats. It was suggested that the dietary VE enhance the ATPase activity by lessen the LPO in membrane. MeSH\ Cold; Erythrocyte membrane; Malondialdehyde; Na(+) K(+)-transporting ATPase