微元素粉末对炎症介质介导的猪冠状循环微静脉高通透性的影响

Effects of microelement powder on the agonist-induced hyperpermeability in porcine coronary venules

目的：本实验旨在观察微元素粉末对炎症介质介导的猪冠状循环微静脉高通透性的影响。方法：利用微血管分离和插管技术，在严格控制微静脉跨膜压力、流量和渗透压的前提下，测量了微静脉对ＦＩＴＣ荧光白蛋白的通透性及微元素粉末对其的影响。结果：微元素粉末对炎症介质组胺介导的高通透性有明显的抑制作用，通透系数（１０－６ｃｍ／ｓ，下同）从９３２±１５６下降到４３８±０６。微元素粉末可使一氧化氮（ＮＯ）供体硝普钠和蛋白激酶Ｃ（ＰＫＣ）激活剂佛波酯醇导致的微静脉通透系数分别从１０１０±１８７下降到４１３±０２４和从８３８±１１０下降到４７３±０６７（Ｐ＜００５）。结论：微元素粉末可能通过阻断ＮＯ和ＰＫＣ信息传导通路。

Abstract AIM: The effect of microelement powder on agonist-induced hyperpermeability in porcine coronary venules is observed.METHOD:The apparent permeability coefficient (Pa, 10 -6 cm/s) to FITC-albumin were measured in isolated and perfused coronary venules[ diameter(54 4±2 4)μm] using a fluorescent ratio technique.RESULTS: The powder solution could inhibit the increased permeability induced by inflammatory mediator histamine with the alteration of Pa from 9 32±1.56 to 4 38±0 65 ( P <0 05).In order to investigate its inhibition mechanism, the effect of powder solution on nitric oxide (NO) donor sodium nitropursside (SNP) and protein kinase C (PKC) activator porbol myristate acetate (PMA) induced hyperpermeability were also detected. SNP, as well as PMA, increased venular permeability two to threefold. The powder solution attenuated this hyperpermeability response significantly with Pa changing from 10 10±1 87 to 4 13±0 24 and from 8 38±1 10 to 4 73±0 67, respectively ( P <0 05).CONCLUSION: The inhibitory effect of powder solution on agonist-induced high permeability in venules might be due to the blocking of NO or PKC signal transduction pathways. MeSH Venules; Permeability; Histamine; Nitric oxide