5/6肾切除大鼠残肾血小板源生长因子-α表达及其意义的探讨

Increased expression of PDGF-α and its meaning in the remnant kidney of 5/6 nephrectomized rats

目的：研究慢性肾衰残余肾组织血小板源生长因子－α（ＰＤＧＦ－α）的表达状况及其机理和意义。方法：采用斑点杂交和形态计量等方法，对假手术对照大鼠、５／６肾切除大鼠、以及５／６肾切除术后给予维生素Ｅ治疗的大鼠肾组织ＰＤＧＦ－αｍＲＮＡ含量及其相关指标进行定量分析。结果：①５／６肾切除大鼠残存肾组织ＰＤＧＦ－αｍＲＮＡ含量显著增高；②５／６肾切除大鼠周围血单个核细胞内游离钙浓度显著增高，细胞内游离钙浓度与ＰＤＧＦ－αｍＲＮＡ含量成正相关；③５／６肾切除大鼠残肾组织有显著的固有细胞增殖、肾小球硬化和肾间质纤维化，肾组织羟脯氨酸含量显著增高，ＰＤＧＦ－αｍＲＮＡ含量与硬化肾小球比率、病变肾小管和肾间质体密度、肾组织羟脯氨酸含量成正相关；④给予维生素Ｅ治疗的大鼠，随着细胞内游离钙浓度和ＰＤＧＦ－αｍＲＮＡ含量降低，残肾硬化肾小球比率、病变肾小管和肾间质体密度、肾组织羟脯氨酸含量显著下降。结论：ＰＤＧＦ－α高表达可能是残存肾组织进行性纤维化的重要原因之一；维生素Ｅ治疗能降低ＰＤＧＦ－α表达，抑制残肾纤维化。

Abstract AIM:To clarify the expression of platelet derived growth factor-α (PDGF-α) and its meaning in the remnant kidney.METHODS:The quantitative assay of PDGF-α mRNA content and relative indices of remnant kidney in 5/6 nephrectomized rats were carried out with dot hybridization, morphometry and so on. RESULTS:① The PDGF-α mRNA content of remnant kidney in 5/6 nephrectomized rats increased significantly compared with the control.②The cytoplasmic free calcium concentration of mononuclear cells in 5/6 nephrectomized rats elevated markedly.The cytoplasmic free calcium concentration correlated positively with the PDGF-α content.③There were obvious hyperplasia of renal inherent cells,and increase of sclerosis glomerulus rate,diseased renal tubular and interstitial volume density,and hydroxyproline content of remnant kidney in 5/6 nephrectomized rats.④In rats received vitamin E treatment,companied with the decrease of PDGF-α content,there was marked alleviation of the histological and pathophysiological damage indices of the remnant kidney.CONCLUSION:High expression of PDGF-α might contribute to the hyperplasia of renal inherent cells and to the glomerular sclerosis and renal interstitial fibrosis.Vitamin E could suppress PDGF-α expression,modify the histological and pathophysiological impairment of remnant kidney. MeSH Platelet-derived growth factor;Kidney;Rat;Renal failure,chronic