重组人白细胞介素-1β对化疗小鼠巨核系造血及存活率的影响

Effects of rhIL-1β on megakaryocytic hematopoiesis and survival of the mice with chemotherapy

目的：观察重组人白细胞介素１β（ｒｈＩＬ－１β），对用细胞毒化疗药物后，机体巨核系造血功能恢复及存活率的影响。结果：①给予不同剂量ｒｈＩＬ－１β（３×１０３～３×１０５Ｕ／鼠）后小鼠腹腔注射半数致死量环磷酰胺，外周血血小板的恢复明显加快，其中以３×１０５Ｕ剂量组效果最佳；注射后５ｄ骨髓ＢＦＵ－Ｍｅｇ含量随给药剂量加大而增加。②给予不同剂量ｒｈＩＬ－１β（３×１０３～３×１０５Ｕ／鼠）后小鼠腹腔注射致死量环磷酰胺，３０ｄ存活率各用药组较对照组均有提高，其中以３×１０５Ｕ组结果最佳（Ｐ＜００５）。结论：ｒｈＩＬ－１β能促进注射半致死量环磷酰胺的小鼠巨核系造血功能恢复，并提高注射致死量环磷酰胺小鼠的３０ｄ存活率。

Abstract AIM and METHOD: To study the effects of rhIL-1β on megakaryocytic hematopoiesis and survival of the mice with chemotherapy, different doses of rhIL-1β were given to C 57 BL/6J inbred strains of mice, wihch were, lately, treated with 200mg/kg or 380mg/kg cyclophosphamide. RESULTS: The recovery of the peripheral platelet counts from 200mg/kg cyclophosphamide were much quicker in the groups of mice pretreated with rhIL-1β than the untreated ones in a dose dependent manner. On the 5th day postinjection BFU-Meg in the bone marrow increased with the increasing dose of rhIL-1β When the dose of cyclophosphamide was increased to 380mg/kg, 30-day survival rate of the group that was pretreated with 3×10 5U rhIL-1β each was significantly elevated ( P <0 05) as compared with the control.CONCLUSION: rhIL-1β promoted the recovery of megakaryocytic hematopoiesis and increased the survival rate of the mice. MeSH Interleukin-1;Cyclophosphamide