急性低氧性肺动脉高压与磷脂酶A_2活力关系初探

The primary study about the relation between acute hypoxic pulmonary arterial hypertension and activity of phospholipase A 2

目的：探讨ＰＬＡ２及相关炎症介质在急性低氧性肺动脉高压形成中的作用。方法：用３０只ＳＤ大鼠随机分为三组（各１０只）：正常对照组（Ａ）、低氧组（Ｂ）、低氧加地塞米松组（Ｃ）。测定磷脂酶Ａ２活力（ＰＬＡ２）、血小板活化因子（ＰＡＦ）、前列腺素Ｅ２（ＰＧＥ２）、血栓素Ｂ２（ＴＸＢ２）。结果：Ｂ组低氧３０ｍｉｎ后，平均肺动脉压（ｍＰＡＰ）、血及肺中ＰＬＡ２活力、ＰＧＥ２、ＴＸＢ２、ＰＡＦ均明显增高；Ｃ组上述指标均明显低于Ｂ组。低氧情况下，ＰＬＡ２与ｍＰＡＰ、ＰＡＦ、ＰＧＥ２、ＴＸＢ２均呈正相关；ＰＡＦ、ＰＧＥ２、ＴＸＢ２分别又与ｍＰＡＰ呈正相关。结论：ＰＬＡ２通过相关炎症介质在急性低氧性肺动脉压的形成中可能起重要的介导作用。

Abstract AIM:The action of phospholipase A 2 (PLA 2) and related inflammatory mediators on the formation of hypoxic pulmonary arterial hypertension was studied.METHODS:30 Sprague-Dawley rats were equally divided into three groups at random:group A was the normal control group; group B was given 10% hypoxia ventilation; group C was pretreated with dexamethasone.RESULTS:After hypoxia, the mean pulmonary arterial pressure (mPAP), the PLA 2 activity, PGE 2, TXB 2 and PAF in blood and lung tissue were increased significantly; but pretreatment with dexamethasone relieved the changes mentioned above. In hypoxia, a positive correlations was found between PLA 2 activity and mPAP, PAF, PGE 2,TXB 2 respectively; positive correlations were also found between PAF, PGE 2, TXB 2 and mPAP.CONCLUSION:PLA 2 plays an important inducing role in the formation of acute hypoxic pulmonary arterial hypertension. MeSH Phospholipases A ; Platelet activating factor; Prostaglandins; Pulmonary artery