健脾清化方对慢性肾衰竭大鼠血清刺激肾小管上皮细胞TGF-β1表达的影响

Effects of Jianpi Qinghua Recipe on the expression of transforming growth factor β1 mRNA in renal tubular epithelial cells stimulated by serum of chronic renal failure rats

目的观察健脾清化方对慢性肾衰竭（CRF）大鼠血清刺激肾小管上皮细胞转化生长因子β1（TGF-β1）mRNA表达的影响。方法模型组和治疗组大鼠予以5／6肾切除法建立CRF模型，并分别予以蒸馏水和健脾清化方灌胃8周。干预结束后，腹主动脉采血，分离血清。体外培养猪近端肾小管上皮细胞株（LLC-PK），分别用含5％、10％的各组大鼠血清的培养液培养，在第8h和24h时用消化液消化并收集细胞，采用逆转录聚合酶链反应（RT-PCR）技术检测各组大鼠。肾组织TGF-β1mRNA的表达水平。结果在血清浓度及培养时间相同的情况下，治疗组肾小管上皮细胞TGF-β1mRNA的表达较模型组降低（P〈0．01）。结论健脾清化方治疗8周后提取的含药血清能明显降低肾组织TGF-β1mRNA的表达，这可能是健脾清化方延缓CRF进展的机制之一。

Objective To evaluate the effects of Jianpi Qinghua Recipe on the transforming growth factor β1 （TGF-β1） mRNA expression of renal tubular epithelial cell stimulated by serum of chronic renal failure（CRF）rats. Methods CRF model was established in model group and treatment group by 5/6 nephrectomy, then distilled water and Jianpi Qinghua Recipe were given respectively by gavage for eight weeks. After eight weeks of treatment, bloods were taken from aorta abdominalis, and serums were made disconnected. Cultured pig proximal tubular epithelial cell line （LLC-PK） were divided into three groups and incubated respectively with 5%, 10% serum of rats in three groups. At 8th and 24th hour, cells were digested with digestion, then to collect cells. To detect the levels of renal tissue TGF-β1 mRNA expression by reverse transcription-polymerase chain reaction method. Results The TGF-β1 mRNA expression was significantly decreased in treatment group compared with that incubated with the same concentration serum and time of in model group（P〈0. 01）. Conclusions Jianpi Qinghua Recipe significantly reduce the renal expression of TGF-β1 mRNA, which may explain one of the mechanisms of delaying the CRF.