摘要
以受体杨树品种‘107号’和转基因杨树‘18-1’的一年生枝条为材料,采用Hoagland营养液水培方法,检测二者植株生长以及根、茎、叶中Na+和K+含量变化的结果表明,在含100mmol·L-1NaCl的Hoagland营养液中,二者的生长速度都受到明显抑制,但后者的受抑程度较小;叶中Na+含量呈持续增加趋势,9d后其叶中的Na+含量显著高于前者。在二者叶片枯萎程度相同的情况下,‘18-1’叶中的Na+含量是‘107号’的1.6倍左右。在4mol·L-1NaCl溶液中,它们的表皮细胞死亡率分别为42%和97%。说明后者比前者有更高的耐盐能力。
Plant growth and changes of Na^＋ and K^＋ concentrations in leaves, stems or roots were investigated in 1-year-old cuttings from receptor poplar ＇ 107＇ （Populus x euramericana ＇Neva＇） and transgenic poplar ＇ 18-1＇, which were exposed to salt stress in Hoagland solution. It was shown that the growth of both poplars were markedly inhibited in Hoagland with 100 mmol.L^-1 NaC1 addition, while ＇18-1＇ was less inhibited than ＇107＇. The concentration of Na^＋ in leaves was keeping increasing, and after 9 day culture Na^＋ concentration in the leaves of ＇ 18-1＇ was greatly higher than that of ＇ 107＇. The accumulation amount of Na^＋ in ＇ 18-1＇ leaves was about 1.6 times higher than that in ＇107＇ when their leaves displayed same necrosis degree under NaC1 stress. The death ratio of epidermal cells was about 42% and 97% in ＇18-1＇ and ＇107＇ in distilled water with 4 mol.L^-1 NaCl addition, respectively. These results indicated that salt tolerance of the transgenic poplar ＇18-1＇ was much higher than that of receptor poplar ＇107＇.
出处
《植物生理学通讯》
CSCD
北大核心
2009年第11期1070-1074,共5页
Plant Physiology Communications
基金
国家重点基础研究发展计划(2006CB100102)
"十一五"国家科技支撑计划(2007BAD67B02)
