真核细胞起始因子4E与胃癌中医证型关系的研究

Study of the relationship between eIF4E and Chinese medical syndromes of gastric cancer

目的通过检测真核细胞起始因子4E(eIF4E)在胃癌中的表达,探讨其表达与胃癌临床病理及中医证型的关系。方法术前确定胃癌患者的中医证型,术后胃癌组织标本石蜡块常规保存,采用免疫组化Envision法检测eIF4E的表达,分析其表达与胃癌临床病理特征及中医证型的关系。结果1)胃癌组织eIF4E蛋白阳性表达率为85.3%(29/34),显著高于癌旁组织58.8%(20/34)(P<0.05)。2)eIF4E的表达与肿瘤发生部位、肿瘤大小、癌组织浸润深度、组织分化程度及Lauren分型均无明显关系(P>0.05),而与胃癌患者的淋巴结转移状态及病理分期呈正相关(P<0.05)。3)eIF4E蛋白在胃癌不同中医证型中存在着差异表达,痰瘀毒结>肝胃不和>脾胃虚寒,其中痰瘀毒结与脾胃虚寒两组间表达差异有统计意义(P<0.05)。结论eIF4E可作为胃癌进展和预后较差的指标;eIF4E的表达差异可能为胃癌不同中医证型的物质基础。

Objective To investigate the relationship between the expression of eIF4E and clinical pathology and Chinese medical syndromes of gastric cancer by determining eIF4E. Methods Determined Chinese medical syndromes of gastric cancer patients before operation, and conventionally preserved the samples of gastric cancer tissue in paraffin blocks. By Envision immunohistochemical method to detect the expression of eIF4E, and analyzed the relationship between the expression of eIF4E and clinical pathology and Chinese medical syndromes of gastric cancer. Results 1 ） The positive rate of elF4E in gastric cancer tissues was 85.3 % （29/34）, and significantly higher than adjacent tissues（ 58.8 % ,20/34） （ P 〈 0. 05）. 2） The expression of eIF4E in gastric cancer tissues had no significant relationship with the tumor location, size, invasion depth, differentiation degree and Lauren classification （ P 〉 0.05 ）, but has positive correlation with metastasis status of the lymph node and the pathological stage （ P 〈 0.05 ）. 3 ） Levers of eIf4E expression in various Chinese medical syndromes were different, tan-yu-du-jie 〉 gan-wei-bu-he 〉 pi-wei-xu-han, and there was a significant difference between tan-yu-du-jie group and pi-wei-xu-han group（P 〈0.05）. Conclusion eIF4E can be taken as an index of progressing and poor prognosis in gastric cancer. The expressional diversity of eIF4E may be a base of different Chinese medical syndromes.