期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

玉米ZmPT6基因的克隆及RNAi表达载体的构建 被引量：2

Cloning of Maize ZmPT6 Gene and Construction of RNAi Expression Vector
下载PDF
导出
摘要 采用PCR方法分别从玉米郑单958基因组DNA及RNA中克隆了菌根共生磷酸盐转运蛋白基因ZmPT6,序列分析表明,ZmPT6基因全长cDNA 1665 bp,包括一个长97 bp的内含子;编码554个氨基酸,属于Pht1家族成员,由12个疏水的跨膜结构域组成。并构建了35S启动子驱动的植物正义、反义及RNAi表达载体,为下一步玉米的遗传转化及ZmPT6基因的功能研究奠定了基础。 The arbuscular mycorrhizal symbiotic phosphate transporter gene ZmPT6 was cloned from Zea mays Zhengdan 958 genomic DNA and RNA by PCR method.Sequences analysis showed that ZmPT6 full-length cDNA 1 665 bp,and contain a intron of 97 bp.ZmPT6 belong to member of Pht1 family,encoded 554 amino acid,and consist of 12 putative hydrophobic transmembrane domains.Three plant transformation vectors,sense vector p2300-35S-ZmPT6,antisense p2300-35S-ZmPT6r,and RNAi vector p2300-35S-ZmPT6RNAi were constructed and so as ,to provide basis of further genetic transformation and function of ZmPT6 gene.
作者 朱先灿 宋凤斌
机构地区 中国科学院东北地理与农业生态研究所 中国科学院研究生院
出处 《华北农学报》 CSCD 北大核心 2009年第5期11-15,共5页 Acta Agriculturae Boreali-Sinica
基金 国家高技术研究发展计划"863"资助项目(2006AA10Z227)
关键词 ZmPT6基因 正义表达载体 反义表达载体 RNAI载体 ZmPT6 gene Sense expression vector Antisense expression vector RNAi vector
分类号 Q785 [生物学—分子生物学]
  • 相关文献

参考文献21

  • 1Heckman D S,Geiser D M, Eiddel B R, et al. Molecular evidence for the early colonization of land by fungi and plants [ J ] . Science, 2001,293 : 1129 - 1133.
  • 2Martin P. Arbuscular mycorrhiza:the mother of plant root endosymbioses [ J ]. Nat Rev Microbiol, 2008,6 : 763 - 775.
  • 3Smith S E, Read D J. Mycorrhizal symbiosis [ M ]. Academic Press, San Diego, 1997.
  • 4Bago B, Pfeffer P E, Shachar-Hill Y. Carbon metabolism and transport in arbuscular mycorrhizas [J]. Plant Physiol, 2000, 124:949 - 958.
  • 5Schachtman D P, Reid R J, Ayling S M. Phosphorus uptake by plants : From soil to cell [ J ]. Plant Physiology, 1998, 116 : 447 - 453.
  • 6Harrison M J, Dewbre G R, Liu J. A phosphate transporter from Medicago truncatula involved in the acquisition of phosphate released by arbuscular mycorrhizal fungi [J]. Plant Cell, 2002,14 : 2413 : 2429.
  • 7Rauseh C, Daram P, Brunner S, et al. A phosphate transporter expressed in arbuscule-containing cells in potato[ J]. Nature, 2001,414:462 - 470.
  • 8Paszkowski U, Kroken S, Roux C, et al. Rice phosphate transporters include an envolutionarily divergent gene specifically activates in arbuscular mycorrhizal symbiosis[J]. Proceedings of the National Academy of Science, USA ,2002,99: 13324- 13329.
  • 9Nagy R, Karandashov V, Chague V, et al. The characterization of novel mycorrhiza-specific phosphate transporters from Lycopersicon esculentum and Solanum tuberosum uncovers functional redundancy in symbiotic phosphate transport in solanaceous species[ J ]. Plant Journal, 2005,42 : 236 - 250.
  • 10Glassop D, Smith S E, Smith F W. Cereal phosphate transporters associated with the mycorrhizal pathway of phosphate uptake into roots [ J ]. Planta, 2005,222 : 688 - 698.

同被引文献147

引证文献2

二级引证文献11

华北农学报
2009年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部