木质素降解菌的筛选及对秸秆的降解研究

Study on the Screening of the Lignin-degrading Fungus and the Degradation of Straws

为筛选木质素高效降解菌,对筛选的方法体系进行了研究。结果表明,利用三种定性筛选平板结合定量的木质素磺酸钙降解的方法,能明显提高筛选效率,从16个菌株中筛选到3株对木质素有较强降解能力的菌株Tp1、Tf1与Ls21。为进一步验证所筛菌株的降解效果,选取其中综合性能较好的Tf1菌株(凤尾菇,Pleurotus sajor-caju),对小麦秸秆与玉米秸秆进行固态发酵试验,并以黄孢原毛平革菌(Phanerochaete chrysosporium)BKMF-1767作参照,结果表明,Tf1对小麦秸秆与玉米秸秆木质素的降解率分别为38.4%与47.7%,均高于参照菌株(分别为32.2%和34.6%)。

To use three kinds of screening plate,and to combine with quantitative degradation test of calcium lignosulphonate,three lignin-degradation strains,TP1,Tf1 and Ls21 were obtained.In order to confirm the degradation effect of the strains,the strain Tf1（Pleurotus sajor-caju）,was selected to do solid substrate fermentation test about the wheat straw and corn straw.And Phanerochaete chrysosporium（BKMF-1767） was made as a reference.The result showed that the degradation rate of Tf1 to the wheat straw and corn was 38.4% and 47.7% respectively,and it was higher than the reference strain（32.2% and 34.6% ）. This research improves the screening method system of lignin-degrading fungus. There is an important significance to expand the resource of lignin-degrading fungus and exploit the straw resource.