中原牡丹品种初级核心种质构建与代表性检验

Construction and Representative Test of Primary Core Germplasms of Tree Peony Cultivars from Central China

以400个中原牡丹品种的形态学和农艺学性状为基本数据,采用花型分组,组内按平方根比例策略确定取样量,并使用类平均聚类法取样,获得了中原牡丹品种初级核心种质,其取样比例占总体样本的30%。经检测,初级核心种质与总体种质的31个性状中仅花期1个性状的多样性指数达到了显著差异水平,初级核心种质多样性指数对总体种质的代表性达99.20%,表型保留比例达到了100%。表明,这个包含120个样本的初级核心种质能在表型性状上很好地代表总体种质的遗传多样性。

Based on the data of morphological and agronomic traits of 400 tree peony cultivars from Central Plains of China,the primary core germplasms,accounting for 30% of the entire accessions,were constructed by UPGMA cluster,the 400 accessions being grouped according to the floral forms and the proportion of square root within groups.The diversity index difference of 31 traits in the primary core collection and the total accessions were tested,and only flowering time presented the significant diversity index difference, indicating that the representation of the primary core collection to the total was up to 99.20% and the ratio of retained phenotype was 100%. This suggested that the primary core collection of 120 accessions could represent the genetic diversity of phenotypic traits for the entire peony varieties.