摘要
筛选比较了不同保存液、保护剂、降温方式、解冻方式对缺帘鱼精液超低温保存活力的影响,初步解释了缺帘鱼精子经超低温短期保存后活力明显较鲜精低的原因。结果显示:Ringer′s液作为保存液,16%二甲亚砜作为保护剂,选取五步法超低温冷冻保存精子[精子缓慢降温至-150℃→液氮面上3 cm(约-170℃),停留4 min→液氮面(约-190℃),停留1 min→液氮];40℃水浴解冻取得最好的冻后活力,解冻后精子活力为(35.2±4.5)%。
In the paper, attempts to develop simple, reliable and efficient methods for cryopreservation are reported. Different methods were used to preserve the sperm, such as, different extenders, cryop rotectants and itsconcentration, freezing rate, thawing rate, etc. The results indicated that using DMSO supplemented with 16% methanol as extender, five-step freezing procedure and 40~C thawing water bath temperature was the best method [ with motility of （35.2 ± 4.5）% ] for the cryopreservation of Brycon cephalus＇ spermatoza.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2009年第4期467-471,共5页
Journal of Jilin Agricultural University
基金
北京市科技计划项目(H020720020230)
关键词
缺帘鱼
精子
超低温保存
Brycon cephalus
spermatozoa
cryopreservation
