越橘品种的ISSR鉴定及其亲缘关系分析

Identification of Blueberry Cultivars and Evaluation of Their Genetic Relationship by ISSR Technique

利用ISSR分子标记对由北美引种到南京的7个南高丛越橘品种(Vaccinium darrowi×V.corymbosum)、9个南高丛越橘实生选择系和12个兔眼越橘(V.ashei)品种共28份材料进行分子鉴别和亲缘关系分析。10个引物共扩增出98条谱带,其中37条为多态性条带,多态率为37.8%。利用其中8个引物扩增的17条多态带可以区分19个品种和9个实生选择系。利用POPGENE1.31软件进行了Nei′s遗传距离计算,利用UPGMA法构建了聚类树状图。聚类结果将各类群单独聚成一大类,证实了它们的亲缘关系。同时显示实生选择系间的遗传差异相对较大,其中7个与南高丛越橘品种聚为一类,而其余2个与兔眼越橘品种聚为另一大类。但是在同一类群内各品种间的遗传关系与谱系数据不完全相吻合,表明本试验所用的ISSR引物不足以用来估算兔眼越橘品种间的遗传关系。

Seven southern highbush blueberry cuhivars （Vaccinium darrowi × V. corymbosum ）, 9 promising selections from southern highbush blueberry seedlings, and 12 rabbiteye blueberry cuhivars （ V. ashi ）, total 28 samples were examined with ISSR technique to construct DNA fingerprinting table to identify these genotypes and evaluate their genetic relationship. A total of 98 bands were obtained through 10 primers, of which 37, 37.8%, were polymorphic. 17 polymorphic bands amplified from 8 premiers were chosen to construct a DNA fingerprinting table to distinguish genotypes. POPGENE1.31 software was used to calculate the Nei＇s genetic distance and a dendrogram was constructed based on UPGMA cluster analysis. Clusters were grouped out separately as expected. It also indicated that the genetic dis- tances among promising selections were relatively higher than those of cultivars. Among the 9 selections, 7 were grouped in highbush blueberry and the other 2 were included in rabbiteye blueberry group. How- ever, estimation of relative genetic distance between cuhivars （genotypes） within 1 group did not agree well with known pedigree data and, thus, it indicated that 1SSR data obtained by primers used in this study did not accurately assess the genetic relationship of cultivars within one group.