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Tween-80并温热对人膀胱癌EJ/ADM的耐药逆转及作用机制

Reversal of drug resistance in the Adriamycin-resistant human bladder cancer cell lines EJ/ADM by Tween-80 in combination with hyperthermia,and its mechanism
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摘要 目的观察Tween-80合并温热对人膀胱癌阿霉素耐药细胞株的逆转作用及其机制。方法采用MTT法进行体外药物逆转实验,免疫细胞化学染色显示处理前后耐药细胞P-糖蛋白表达情况。结果①Tween-80合并41℃温热作用前后,EJ/ADM对抗癌药物阿霉素、丝裂霉素、5-氟尿嘧啶等药物的敏感性显著增加(P<0.01);②Tween-80合并41℃温热能够协同抑制p-gp蛋白的表达。结论Tween-80合并41℃温热对人膀胱癌阿霉素耐药细胞的耐药性有逆转作用,逆转机制与其抑制p-gp蛋白的表达有关。 Objective To investigate the reversal of drug resistance in the ADM-resistant human bladder cancer cell lines EJ/ADM by Tween-80 in combination with hyperthermia, and its mechanism. Method The reversing drug resistance was studied by MTT and the expression of P-glyeoprotein were examined by immunocytochemistry. Result MTT eytotoxic assay revealed that the sensitivities of EJ/ ADM cells to ADM, MMC, 5-FU, HCPT, VCR were different according to presence or absence of Tween80 combined with hyperthermia （41℃）（P〈0.01）. The immuno-cytochemistry showed that the P- glycoprotein distributes in cellular membrane and cytoplasm, and after treated with Tween80 combined with hyperthermia （41℃）, P-glycoprotein staining was redused in EJ/ADM cell. Conclusion The drug resistance of EJ/ADM cells could be reversed by Tween-80 in combination with hyperthermia（41℃）.
作者 刘迪 朱玉芬
机构地区 上海市徐汇区中心医院肿瘤科
出处 《实用医药杂志》 2009年第11期64-66,共3页 Practical Journal of Medicine & Pharmacy
关键词 TWEEN-80 温热 EJ/ADM 多药耐药 P-GP Tween 80 Hyperthermia EJ/ADM Drug resistance P-glycoprotein
分类号 R734.2 [医药卫生—肿瘤]
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实用医药杂志
2009年 第11期

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