基于纳米银对鲁米诺-铁氰化钾化学发光体系的增敏作用测定硫酸特布他林

Determination of Terbutaline Sulfate Based on the Enhancement Chemiluminescence of Ag Nanoparticles in Luminol-Potassium Ferricyanide Chemiluminescence System

基于纳米银对鲁米诺-K3Fe(CN)6化学发光体系的显著增敏作用,建立了流动注射化学发光检测硫酸特布他林的新方法。运用透射电子显微镜(TEM)对所制备的纳米银进行了表征。化学发光光谱和紫外吸收光谱显示将纳米银注入鲁米诺-K3Fe(CN)6体系后,未生成新的发光物质。考察了鲁米诺,K3Fe(CN)6,NaOH以及纳米银浓度对化学发光体系的影响,在优化的实验条件下,该方法对硫酸特布他林检测的线性范围是1.0×10-9～2.0×10-5g/L(r=0.9935),检出限为1×10-10g/L,相对标准偏差为3.6%(C=1.0×10-6g/L,n=11)。本方法应用于硫酸特布他林片剂含量的测定,回收率为98.5%～102.5%,并与药典方法进行了比较,结果基本一致。

It was found that Ag nanoparticles （NPs） could enhance the chemiluminescence （CL） intensity of luminolpotassium ferricyanide system. On the basis of enhancement effect, a flow injection method was developed for the determination of terbutaline sulfate. The structure and shape of Ag NPs were characterized by transmission electron microscopy. The UV-Vis absorption spectra and chemiluminescence spectra suggested that new luminophor was not formed after Ag NPs introducing in luminol-potassium ferricyanide chemiluminescence system. A possible mechanism of Ag NPs strengthening on luminol-potassium ferricyanide CL reaction was also discussed. The effect of concentration of luminol, potassium ferricyanide, sodium hydroxide and Ag NPs on CL reaction was investigated respectively. In the optimum conditions, the linear range was 1.0 ×10^-9 _ 2.0×10^-5 g/L（r =0. 9935） and the detection limit was 1 × 10^-10 g/L. The relative standard deviation （RSD） was 3.6% for 1.0 × 10^-6 g/L terbutaline sulfate （n = 11 ）. The recommended method has been successfully applied to the determination of terbutaline sulfate tablets and the recovery was between 98.5% - 102.5%, moreover the results were almost identical with the same results of Pharmacopoeia method.