α-萘基缩水甘油醚对映体的毛细管区带电泳手性拆分

Chiral Separation of Naphthylglycidic Ether Using Capillary Zone Electrophoresis

建立了毛细管区带电泳手性拆分α-萘基缩水甘油醚对映体的方法。考察了不同手性拆分试剂对手性选择性的影响,实验结果表明,20mmol/L H3PO4-三乙醇胺(pH2.5)、2%(w/V)HS-β-CD、毛细管温度20℃、运行电压-18kV为最佳分离条件,在该分离条件下α-萘基缩水甘油醚对映体实现基线分离。方法简便、准确,可用于α-萘基缩水甘油醚的手性拆分和对映体过量值(ee,%)测定。

A capillary electrophoretic method was developed for the separation of enantiomers of naphthylglycidic ether（NGE）. Several cyclodextrins（CDs） were applied as the chiral selectors and it was found that the ionic modified highly sulfated eyclodextrin（HS-β-CD） could give satisfactory enantioselectivity. In addition, the effects of the pH value of the buffer system, the concentration of the HS-β-CD, capillary temperature and the running voltage on the chiral separation were investigated systematically. The result showed that under the optimized conditions of pH 2.5, 20 mmol/L H3PO4-triethanolamine buffer ,containing 2% highly sulfated cyclodextrin （HS-β-CD） at capillary temperature 20 ℃ in the reversed polarity voltage of - 18 kV, the enantiomers of naphthylglycidic ether were baseline separated. This method is simple, precise and can be applied to the ehiral separation of naphthylglycidic ether enantiomers and determination of enantiomers excess （ee, % ）.