葫芦素B水-醇凝胶的体外经皮通透性

In vitro transdermal permeability of cucurbitacin B hydro-alcohol gels

目的考察处方因素对葫芦素B水-醇凝胶的体外通透性的影响。方法建立了HPLC法测定葫芦素B的方法。采用Franz扩散池,以大鼠离体皮肤为通透膜,研究葫芦素B水-醇凝胶体外经皮通透性。结果选择的3种通透促进剂中,质量分数(w)为1%的油酸溶液具有最佳的促透效果,含葫芦素B质量分数为0.2%的凝胶经大鼠皮肤24 h累积通透量Qt达(410.39±33.75)μg.cm-2。结论水-醇凝胶是葫芦素B经皮给药的有效载体。

Objective To investigate the effect of various factors on the in vitro transdermal penetration of cucurbitacin B hydro-alcohol gels. Methods An HPLC method was established for the assay of cucurbitacin B. The percutaneous permeation of cucurbitacin B from hydro-alcohol gels was investigated using excised rat skin as model membrane with Franz diffusion cells devices. Results Of the three penetration enhancers used in this experiment, 1% OA （ oleic acid） showed the optimum enhancement effect, the accumulated amount of drug penetrated through rat skin at 24 h was （410. 39 ±33.75 ）μg·cm^-2（ 0. 2% cucurbitacin B gel）. Conclusions Hydro-alcohol gel can be an effective cartier for transdermal delivery of cucurbitacin B.