新疆哈萨克族β-纤维蛋白原-455G/A基因多态性与原发性高血压的关系——附80例报告

Relationship between the polymorphism of β-fibrinogen gene-455G/A and essential hypertension in Kazakh of Xinjiang

目的:探讨新疆哈萨克族人群β-纤维蛋白原-455G/A基因多态性与原发性高血压(essential hypertension,EH)的关系。方法:应用聚合酶链反应-限制性片段长度多态性技术(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP),对80例新疆哈萨克族EH患者(EH组)及103名健康体检者(对照组)进行β-纤维蛋白原-455G/A基因多态性分析。结果:β-纤维蛋白原-455G/A基因多态性在被检哈萨克族人群中的分布符合Hardy-Weinberg平衡定律。EH组的GG、GA、AA基因型分布频率为55%、41%、4%,对照组为60%、38%、2%,2组比较差异无统计学意义(P>0.05);EH组的A等位基因频率为24%,略高于对照组的21%,但2组比较差异无统计学意义(P>0.05)。结论:新疆哈萨克族EH患者和健康人均存在β-纤维蛋白原-455G/A基因的多态现象,提示β-纤维蛋白原-455G/A基因多态性可能不是新疆哈萨克族EH的遗传易感标志。

Objective： To investigate the relationship between the polymorphism of β-fibrinogen gene- 455G/A and essential hypertension（EH）in Kazakh of Xinjiang. Methods： Polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP） was used to detect the 455G/A polymorphism of β-fibrinogen gene in Kazakh of Xinjiang, including 80 cases of EH patients（EH group） and 103 cases of normotensives （normal group）. Results： 455G/A polymorphism of β-fibrinogen gene was conformed to Hardy-Weinberg equilibrium in investigated Kazakh. The genotype frequencies of GG, GA and AA were 55%, 41% and 4% in EH group, and 60% , 38% and 2% in normal group, respectively. There was no significant difference between the two groups（P 〉0. 05）. Although the A allele frequencies were higher in EH group（24% ） than in normal group（21% ）, but there was no statistical significance between the two groups （ P 〉 0.05 ）. Conclusion ： There is polymorphism of β- fibrinogen -455G/A gene in EH patients and normotensives of Xinjiang. It suggests that the β-fibrinogen-455G/A gene polymorphism may not be the genetic susceptibility to EH in Kazakh of Xinjiang.