广西沿海地区Graves病与HLA-DQA1、DQB1DRB1*09等位基因的相关性研究

The association between Graves'disease and polymorphism in HLA-DQA1,DQB1 and DRB1*09 alleles in Guangxi coastal area

目的研究广西沿海地区Graves病(graves disease,GD)患者中HLA-DQA1、DQB1及DRB1*09等位基因的分布情况及其关系。方法采用序列特异性引物PCR(PCR-SSP)方法检测162例广西沿海地区GD患者及90例健康者的HLA-DQA1、DQB1及DRB1*09的基因型,计算和比较各组间等位基因频率。结果与健康对照组比较,HLA-DQA1*0501、DQB1*0303和HLADRB1*0901在GD患者组的分布频率明显增高(P<0.05);GD女性患者组中的HLA-DQA1*0301频率比健康对照组女性明显升高,差异有统计学意义(P<0.05)。结论HLA-DQA1*0501、HLADQB1*0303及HLA-DRB1*0901是广西沿海地区GD遗传易感基因;DQA1*0301是广西沿海地区女性GD患者中的遗传易感基因。

Objective To study the distribution of HLA DQA1, DQB1 and DRB1 ＊ 09 alleles in patients with Graves＇dlsease （GD） in Guangxi coastal area as well as their association. Methods HLA-DQA1, DQB1 and HLA-DRB1 ＊ 09 alleles were typed by PCR amplification with sequence-specific primers （PCR SSP） method in 162 GD patients and 90 normal subjects. The comparison of allele frequencies was made between GD group and healthy control group. Results The gene frequencies of HLA-DQA1 ＊ 0501, DQB1 ＊ 0303 and DRB1 ＊ 0901 were significantly higher in GD group than thoes in healthy control group （P〈0.05） In GD group, the frequency of HLA DQA1 ＊ 0301 was markedly higher in female subgroup than that in male subgroup （P〈0.05）. Conclusion HLA DQA1 ＊ 0501, DQB1 ＊ 0303 and DRB1 ＊ 09 are predisposing genes of GD in GuangXi coastal area. HLA-DQA1 0301 allele is the predisposing gene of female GD in the area.