利用小动物活体成像技术快速评价呼吸道给药效果

Rapid Assessment of Respiratory Drug Delivery Efficacy with Small Animal Imaging Technology

目的:通过小动物活体成像观察荧光物质在动物呼吸道的分布,以快速评价呼吸道给药效果。方法:昆明种小鼠鼻腔吸入给予Cy5.5荧光标记的脂质基因复合物气雾剂设为实验组,同时设立阳性对照组,采用气道插管方式注入与实验组等量复合物无水乙醚溶液,阴性对照组吸入给予等量不含Cy5.5标记的气雾剂,观察气雾颗粒在各组小鼠呼吸道的荧光分布。结果:与阴性对照组比较,实验组和阳性对照组小鼠的呼吸道内均有强烈的荧光显示。结论:小动物活体成像技术可能成为呼吸道给药效果的快速、有效的评价方法。

OBJECTIVE： To observe the distribution of fluorescent material in the respiratory tract of animals with the use of small animal imaging technology to quickly evaluate the effects of respiratory drug delivery. METHODS： The Kunming mice in the experimental group were administered with CyS. 5 fluorescent- labeled aerosol, meanwhile, a positive control group was set up by injecting same amount of compound anhydrous diethyl ether solution via tracheal incubation, with negative control group inhaled the same amount of non- CyS. 5 fluorescently- labeled aerosol. The fluorescent distribution of the aerosolized particles in the respiratory tract was observed. RESULTS： Fluorescentlylabeled aerosol was observed in both the experi- mental group and the positive control group but not in negative control group. CONCLUSION： The small animal imaging technology is expected to be used as a rapid and effective method for evaluating the efficacy of respiratory drug delivery.