Survivin、PTEN和Cx43基因在皮肤基底细胞癌中的表达

The Expressions of Survivin,PTEN and Cx43 Gene in Skin Basal Cell Carcinoma

背景与目的:探讨Survivin、PTEN和Cx43基因与皮肤基底细胞癌(basal cell carcinoma,BCC)的相关性。材料与方法:采用免疫组织化学S-P法分别检测35例皮肤BCC、10例正常皮肤组织中Survivin、PTEN和Cx43蛋白的表达;运用核酸分子原位杂交技术检测Cx43 mRNA的表达。应用图像分析系统采集分析图像,分别检测BCC和正常皮肤组织中各项检测指标的表达水平(阳性面积)和表达强度(光密度)。结果:Survivin蛋白在正常皮肤中呈阴性表达,在BCC中呈弱阳性表达,其差异有统计学意义(P<0.05);PTEN蛋白在正常皮肤组织中呈强阳性表达,在BCC中呈阳性或弱阳性表达,其差异具有统计学意义(P<0.05)。而Cx43蛋白和Cx43 mRNA在正常皮肤组织中的表达均呈强阳性,而在BCC组织中均呈阳性或弱阳性,差异均具有统计学意义(P均<0.01)。相关性分析显示,BCC组织中,PTEN蛋白的表达与Cx43蛋白呈正相关(r=0.519,P<0.01),Cx43蛋白的表达与Cx43 mRNA也呈正相关(r=0.732,P<0.01)。结论:PTEN蛋白、Cx43蛋白和Cx43 mRNA在BCC中的低表达;Survivin蛋白在BCC中的高表达,可能在BCC发生发展的过程中发挥重要作用。

[ABSTRACTJ BACKGROUND AND AIM： To investigate the relationships of Survivin, PTEN and Connexin43（Cx43） with development of skin basal cell carcinoma（BCC）.MATERIALS AND METHODS： The expressions of Survivin, PTEN and Cx43 proteins were measured by immunohistochemical S-P method in 35 patients with BCC and 10 specimens of normal skin. The expressions of Cx43 mRNA was evaluated by in situ hybridization.All data in normal epidermis and BCC tissues were analyzed by computerized image-analysis. RESULTS： Cx43 protein and its mRNA showed strongly positive expression in normal epidermis,and positive or weakly positive expression in BCC group. Compared with normal skin group and SCC group, the expression（positive area, optical density） of Cx43 protein and its mRNA in BCC group were statistically significant（P〈0.01） Survivin protein was mostly negative in normal epidermis, and mostly weakly positive in BCC group.The expression of Survivin protein was statistically different between BCC and normal skin（P 〈 0.05） PTEN protein showed strongly positive expression in normal epidermis,and positive or weakly positive expression in BCC group. Compared with normal skin group, the expression of PTEN protein in BCC group was statistically significant（P 〈 0.05） . There was positive correlation between the expressions of PTEN and Cx43 protein（r = 0.519, P 〈 0.01）, and between the expressions of Cx43 protein and its mRNA（r = 0.732,P 〈 0.01） in BCC tissues. CONCLUSION： The lower expression of Cx43, PTEN proteins and Cx43 mRNA and the higher expression of Survivin protein in BCC may play important roles in its development.