囊泡膜谷氨酸转运体与ASIC3或TRPV1在大鼠结状神经节内的共存研究(英文)

Co-localization of vesicular glutamate transporters with ASIC3 or TRPV1 in the nodose ganglion of rat

为了探讨囊泡膜谷氨酸转运体(VGluTS)与酸敏感离子通道亚基3(ASIC3)或瞬时感受器电位香草酸亚型1(TRPV1)样阳性产物在大鼠迷走神经结状神经节(nodoseganglia,NG)内的分布和共存,本研究采用免疫荧光组织化学双重标记技术,在激光共聚焦显微镜下观察了大鼠NG内VGluT1或VGluT2分别与ASIC3或TRPV1的共存情况。结果显示:(1)在NG内,VGluT2、ASIC3和TRPV1主要见于中、小型神经元的胞浆内,大型神经元少见,而VGluT1阳性神经元数量较少,主要见于大型或中型神经元;(2)部分VGluT2阳性神经元同时显示ASIC3或TRPVl免疫活性,其中VGluT2/ASIC3双标神经元分别占VGluT2阳性神经元的43.06%,占ASIC3阳性神经元的58.74%;而VGluT2/TRPVl双标神经元分别占VGluT2或TRPVl阳性神经元的5617%和51.12%;(3)VGluTI与ASIC3或TRPV1双标神经元的数量很少,不到1%。以上结果提示,VGluT2主要存在于NG内中、小型神经元,这些神经元通常被认为是内脏伤害性信息的初级感受神经元,因而VGluT2分别与ASIC3或TRPVl的共存表明它们可能与内脏伤害性信息的产生和传递密切相关。

To investigate the distribution of vesicular glutamate transporters （VGluTs） and their co-existence with acid sensing ion channel subunit 3 （ ASIC3 ） and transient receptor potential vanilloid 1 （ TRPV1 ） in the nodose ganglion （NG） of the vagus nerve of rat, the present study used the histochemical double-labeling technique to observe the co-localization of VGluT1 or VGluT2 with ASIC3 or TRPV1 in the NG of rats under a confocal laser-scanning microscope. The results showed that： （ 1 ） In the NG, VGIuT2, ASIC3 and TRPV1 mainly existed in the cytoplasm of small to medium-sized neurons, a few in the large-sized neurons. While the number of VGIuTI -immunopositive neurons was a little and mainly be observed in the large on medium-sized neurons; （2） Some of the VGluT2-immunoreactive （-ir） neurons showed simultaneously ASIC3- or TRPV1-immunoreactivity. The VGluT2/AS1C3 double-labeled neurons were about 43.06% or 58.74% among VGluT2- or ASIC3-ir neurons respectively. On the other hand, about 56.17% or 51.12% of VGluT2- or TRPV1-ir neurons were dually labeled with VGIuT2/TRPV1 ; （3） A few of VGluT1-ir neurons were double-labeled with ASIC3 or TR- PV1 in the NG （less than 1% ）. The present results indicate that in the NG, VGluT2-immunoreactivity mainly localizes in small- to medi- um-sized neurons which were known as the primary afferent neurons of the visceral nociceptive sensing. The co-existence of VGluT2 with ASIC3 or TRPV1 suggest that they might be involved in the produce and transmission of the visceral nociceptive sensation information.