摘要
我们检验了在在盐下面的小白菜的应答的基因强调并且分析的盐忍耐他们的潜在的功能。mRNA 微分显示被用来屏蔽抄本与在容忍、中等容忍的小白菜的咸度忍耐有关的导出的碎片(TDF ) 细菌原生质。78 教材联合产生了 101 微分 cDNA 碎片，它被划分成 10 种表示类型。获得并且定序的七个 cDNA 序列(GenBank 就职 Nos. DQ006915∼DQ006921 ) 对与在在不同不能生活的应力下面的植物的发信号的小径有关的一些已知的表示基因或基因高度相应。
We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The LRNA differential display was used to screen the transcript derived fragments （TDFs） related to salinity tolerance in tolerant and Loderately tolerant Pak-choi germplasm. Seventy-eight primer combinations generated 101 differential eDNA fragments, which ere divided into 10 expression types. Seven cDNA sequences （GenBank accession Nos. DQ006915-DQ006921） obtained and ,~quenced were highly homologous to some known expression genes or the genes related to the signaling pathways in plants under ifferent abiotic stress.
基金
Project supported by the National "the Tenth Five-Year-Plan" Key Program (No. 2004BA525B08)
China and the Key Laboratory of Vegetable Genetics and Physiology, Ministry of Agriculture, China
关键词
差异表达基因
耐盐性
过磷酸钙
风险值
油菜
GENBANK
芦苇
免疫核糖核酸
Brassica campestris L. ssp. chinensis （L.） Makino var. communis Tsen et Lee, Salinity tolerance, Gene differential expression, cDNA fragments, Basic local alignment search tool （BLAST） analysis
