脂联素基因SNP276多态性与儿童青少年肥胖的相关性研究

The relationship between SNP276 polymorphism of adiponectin gene and obesity in children and adolescents

目的研究脂联素(APM1)基因SNP276 G/T多态性与儿童青少年单纯性肥胖及其代谢指标的相关性。方法以2004至2006年于复旦大学附属儿科医院内分泌门诊就诊的单纯性肥胖或超重儿童青少年分别作为肥胖组和超重组;选择某中学正常体重学生作为正常对照组。分别测量身高和体重,计算体重指数(BMI)。测定血清空腹葡萄糖(FPG)、空腹胰岛素(FIns)、三酰甘油(TG)和总胆固醇(TC)水平。计算胰岛素抵抗指数(HOMA-IR)和胰岛素敏感指数(QUICKI)。抽提外周血基因组DNA,采用Taqman-MGB探针技术检测APM1基因SNP276 G/T多态性,分析不同基因型与代谢指标和BMI间的关联性。结果肥胖组纳入227例,超重组纳入231例,正常对照组纳入216名。①肥胖+超重组的BMI、FPG、FIns、TG和HOMA-IR均显著高于正常对照组。②基因分布频率符合Hardy-Weinberg平衡。③肥胖组、超重组和正常对照组的G等位基因频率分别为71.4%、72.5%和69.7%,GG基因型频率分别为50.2%、52.4%和45.8%,GT基因型频率分别为42.3%、40.3%和47.7%;各组差异均无统计学意义(P均>0.05)。④SNP276 GG、GT和TT基因型的BMI、FPG、FIns、TG、TC、HOMA-IR和QUICKI差异均无统计学意义(P=0.49～0.99)。⑤肥胖+超重组IFG儿童青少年中GG+GT型有70例,TT型4例;正常对照组IFG儿童青少年中GG+GT型有10例,TT型0例;两组差异无统计学意义(P=0.45)。结论APM1基因SNP276 G/T多态性与青少年儿童单纯性肥胖及其代谢指标间无显著关联性,提示该SNP位点可能存在种族特异性。

Objective To investigate the association of SNP276 polymorphism of adiponectin gene with obesity and relative trails in children and adolescents. Methods Obese/overweight children and adolescents and normal weight controls aged 6 - 18 years were recruited. Body height, weight were measured and body mass index（BMI） was calculated. Serum fasting plasm glucose （FPG） , fasting insulin （Fins） , triglycerid（TG） and cholesterol（TC） were measured. HOMA-IR and QUICKI were calculated. Genomic DNA was extracted from peripheral blood and Taqman-MGB probe was used to detect the adiponection （ APM1 ） gene SNP276 G/T polymorphism. Obesity was evaluated using IOTF BMI standard. Results Obese group, overweight group and normal group included 227, 231 and 216 children, respectively. （1）FPG, Fins, TG and HOMA-IR in obese ＋ overweight group were significantly higher than those in normal control group. （2）The G allele frequency of SNP276 was 69. 7% which didn＇t exist significant statistically difference compared with obesity （ allele frequency 71.4% ） and overweight （ allele frequency 72. 5% ） groups（P =0. 678, 0. 550）. （3）There was significant statistically difference in FPG,FIns,TG,TC, HOMA-IR and QUICKI among three SNP276 genotypes （P =0. 49 -0. 99）. （4）Among all the IFG children, 15.3% （70/458） children were GG ＋ GT and 0. 9% （4/458） were Tr genotype in obesity and overweight groups which didn＇t exist significant statitically difference compared with normal control group（4. 6% GG ＋ GT genotype, 0 TT genotype） . Conclusions APM1 gene SNP276 was not associated with BMI and their metabolic parameters in Chinese obesity/overweight children, which suggested that APM1 gene SNP276 polymorphism may exist ethic difference in its relation with obesity and metabolic syndrome.