摘要
选择了一株酿酒酵母菌(Saccharomyces cerevisiae)As2.399,通过单因素和正交实验得到酿酒酵母产乙醇脱氢酶(Alcohol Dehydrogenase,ADH)的最优发酵条件。结果表明:产ADH的最优培养条件是温度为25℃、pH为5、通气量为250mL、接种量为7%,液体发酵培养中pH对ADH活性的影响最大。
A strain yeast Saccharomyces cerevisiae As2.399 was selected to produce ADH.The fermentation conditions of yeast were optimized with single factor and orthogonal test.The result showed that high quality ADH could be produced at the temperature 25℃,the initial pH5.0,medium volume 250mL and the inoculums 7% （v /v）.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第11期176-177,190,共3页
Science and Technology of Food Industry
基金
湖北省国家科技合作重点项目计划(006CA013)
黄石理工学院院级项目(08yjz13Q)
关键词
酿酒酵母
乙醇脱氢酶
发酵
Saccharomyces cerevisiae
alcohol dehydrogenase
fermentation
