响应曲面法优化从桑叶中酶法提取芦丁新工艺

Optimization of enzymatic extraction process of rutin from mulberry (Morus alba L.) leaves using response surface methodology

以桑叶为原料,建立酶法提取芦丁的新工艺。以芦丁提取率为评价指标,单因素法获取酶解工艺参数的优化区间,响应曲面法优化酶法提取芦丁的工艺参数。实验结果表明,从桑叶中酶法提取芦丁的最佳工艺条件为:酶解温度30℃,纤维素酶浓度0.5mg/mL,酶解介质pH3.0,处理时间7h。酶法从桑叶中提取芦丁的平均提取率为0.786mg/g,相比传统乙醇提取法提高了60.5%。

Using mulberry （Morus alba L.） leaves as raw material,adopting an enzymatic extraction method to study a new production process of rutin.Extraction yield of rutin was taken as the evaluation index,optimization range of the enzymatic extraction process parameters were selected using single factor methodology,then those best conditions were optimized using response surface methodology.Experiment results showed that when the enzymatic hydrolysis temperature was 30℃,the concentration of enzyme was 0.5mg /mL, the pH value was 3.0, and the enzymatic treatment time was 7h, the optimum extraction rate of rutin from mulberry leaves was 0.786mg/g, which was 60.5% higher than that in traditional alcohol extraction method.