摘要
目的:建立高效液相色谱法测定人血浆中贝凡洛尔的浓度。方法:空白血浆加贝凡洛尔和内标普萘洛尔,液液萃取法进行提取,然后用HPLC进行分析。采用Plastisil ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-10 mmol甲酸胺水溶液(含0.2%甲酸)(35∶65),流速为1 mL.min-1,检测波长为280nm,柱温40℃,进样量为20μL。结果:本方法线性范围是15.5-1 984 ng.mL-1,r2=0.999 9,最小检出浓度(LLOQ)为15.5 ng.mL-1,绝对回收率均在86%以上,相对回收率在94%-98%之间,日内、日间RSD均小于15%。结论:本方法经济、简便、灵敏,适用于贝凡洛尔血药浓度检测和药动学研究。
Objective:To establish a HPLC method for determination of bevantolol in human plasma.Methods:Liquid-liquid extraction was adopted after the addition of bevantolol and propranolol(an internal standard) into the blank plasma.The analysis involved a Plastisil ODS(250 mm×4.6 mm,5 μm) column.The mobile phase consisted of acetonitrile:10 mmol methanoic acid amine water solution(including 0.2% formic acid)(35∶65);the flow rate was 1 mL·min-1.The detect wavelength was 280 nm,and the column temperature was set at 40℃ Results:The peak area of bevantolol in plasma showed good linearity within the range of 15.5 - 1 984 ng-mL-1, and r2 = 0. 999 9. The LLOQ of bevantolol in plasma was 15.5 ng·mL-1 The extracted recovery was 〉 86% ;the relative recovery was 94% - 98% ; the intra-and inter-day RSD were 〈 15%. Conclusion:This method is sensitive, simple and accurate;it can be used for determination of bevantolol plasma concentration and its pharmacokinetic and bioequivalence study.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2009年第22期2160-2162,2182,共4页
Chinese Journal of New Drugs