快速灵敏的HPLC方法检测人血浆中贝凡洛尔的浓度

A rapid and sensitive HPLC method for determination of bevantolol in human plasma

目的：建立高效液相色谱法测定人血浆中贝凡洛尔的浓度。方法：空白血浆加贝凡洛尔和内标普萘洛尔,液液萃取法进行提取,然后用HPLC进行分析。采用Plastisil ODS柱（250 mm×4.6 mm,5μm）,流动相为乙腈-10 mmol甲酸胺水溶液（含0.2%甲酸）（35∶65）,流速为1 mL.min-1,检测波长为280nm,柱温40℃,进样量为20μL。结果：本方法线性范围是15.5-1 984 ng.mL-1,r2=0.999 9,最小检出浓度（LLOQ）为15.5 ng.mL-1,绝对回收率均在86%以上,相对回收率在94%-98%之间,日内、日间RSD均小于15%。结论：本方法经济、简便、灵敏,适用于贝凡洛尔血药浓度检测和药动学研究。

Objective：To establish a HPLC method for determination of bevantolol in human plasma.Methods：Liquid-liquid extraction was adopted after the addition of bevantolol and propranolol（an internal standard） into the blank plasma.The analysis involved a Plastisil ODS（250 mm×4.6 mm,5 μm） column.The mobile phase consisted of acetonitrile：10 mmol methanoic acid amine water solution（including 0.2% formic acid）（35∶65）;the flow rate was 1 mL·min-1.The detect wavelength was 280 nm,and the column temperature was set at 40℃ Results：The peak area of bevantolol in plasma showed good linearity within the range of 15.5 - 1 984 ng-mL-1, and r2 = 0. 999 9. The LLOQ of bevantolol in plasma was 15.5 ng·mL-1 The extracted recovery was 〉 86% ;the relative recovery was 94% - 98% ; the intra-and inter-day RSD were 〈 15%. Conclusion：This method is sensitive, simple and accurate;it can be used for determination of bevantolol plasma concentration and its pharmacokinetic and bioequivalence study.