吸烟及戒烟对博莱霉素肺间质纤维化大鼠肺组织炎症及纤维化变化的影响

Effects of smoking and smoking cessation on changes of inflammation and fibrosis in lung tissue of pulmonary interstitial fibrosis rats induced by Bleomycin

目的探讨吸烟、戒烟对博莱霉素(BLM)诱导的大鼠肺间质纤维化模型肺纤维化程度、炎症及氧化应激反应的影响。方法选健康Wistar大鼠56只,随机分为空白组;BLM组(A组):气管内滴注博莱霉素组;吸烟BLM组(B组):博莱霉素干预后吸烟;吸烟-戒烟-BLM组(C组)。经吸烟戒烟后,气管内滴注博莱霉素,每组14只。各组随机分别于气管内滴注博莱霉素,14和28d各处死7只。收集肺组织作切片行HE及Masson染色,判断肺组织肺泡炎及肺纤维化程度,测定血清丙二醛(MDA)含量。结果①成功建立博莱霉素诱导的肺间质纤维化模型,各指标在不同时间及组别出现变化。②BLM组28天大鼠肺组织与BLM组14天比较,纤维化反应重、炎症反应轻、MDA含量低。③吸烟BLM组比同期BLM组肺组织纤维化程度较轻、炎症反应及MDA高。④吸烟-戒烟-BLM组比正吸烟BLM组肺组织纤维化程度重、MDA高。结论吸烟可加重肺间质纤维化大鼠肺组织的炎症、氧化应激反应,但同时减轻或延缓纤维化改变。

[ Objective ] To investigate effects of smoking and smoking cessation on fibrosis, inflammation and oxidative stress in lung tissue of pulmonary interstitial fibrosis rats induced by Bleomyein. [Methods] 56 healthy Wistar rats were randomly divided into four groups, control group; Bleomyein（BLM） group：intratraeheal Bleomycin instillation; smoking BLM group：smoking after Bleomycin intervention; smoking-smoking cessation-BLM group： intratraeheal Bleomycin instillation after smoking-smoking cessation, 14 rats each group. Seven rats in each group were executed at days 14 and 28 after intratracheal bleomycin instillation, collecting lung tissue slice for HE and Masson stain, determining lung tissue alveolitis and pulmonary fibrosis, observing the lever of malondialdehyde （MDA） in blood serum. [ Results ] The success of the establishment of Bleomycin-induced pulmonary interstitial fibrosis model. The indicators change at corresponding groups and times. Compared with 14 days, the rat lung tissue of BLM group 28 days have heavier fibrosis, lighter inflammatory response,lower MDA. Compared with BLM group, smoking BLM group have lighter fibrosis, heavier inflammatory response,higher MDA. Compared with smoking BLM geoup,smoking-smoking cessation-BLM grpup have heavier fibrosis,higher MDA. [ Conclusion ] Smoking aggravated inflammation and oxidation stress in pulmonary interstitial fibrosis rat lung tissue, but at the same time to reduce or delay fibrosis change.