联合检测外周血端粒酶活性及组织多肽特异性抗原对乳腺癌临床价值的探讨

Clinical value of diagnosis and treatment of breast cancer of the detection combining telomerase activity with TPS lever

目的探讨联合检测外周血端粒酶活性和组织多肽特异性抗原对乳腺癌患者的临床价值。方法采用聚合酶链反应(PCR)-端粒酶重复序列扩增反应(TRAP)-酶联免疫吸附测定(ELISA)法,分别检测67例乳腺癌患者、31例乳腺良性病变患者及36例体检健康者的外周血单个核细胞的端粒酶活性;同时对其血清应用ELISA法检测组织多肽特异性抗原的表达情况。结果外周血端粒酶活性和组织多肽特异性抗原的表达均与乳腺癌淋巴结转移密切相关(P<0.05)。其中,外周血端粒酶活性的表达还与癌组织的浸润相关(P<0.001)。结论外周血端粒酶活性和组织多肽特异性抗原的表达预示乳腺癌淋巴结转移,并且外周血端粒酶活性的表达还与乳腺癌的局部浸润生长有关。联合检测这2项指标,对患者预后的评估及治疗方案的制定具有重要的意义。

[ Objective ] To study the expression of telomerase activity and TPS lever in diagnosis and treatment of breast cancer. [ Methods ] We have detected telomerase activity and TPS lever of 67 cases of breast cancer and 31 cases of breast benign diseases and 36 cases of healthy men by PCR-TRAP-ELISA and ELISA. [ Results ] The expression of telomerase activity and TPS lever were all correlated to tumor metastasis （P 〈0.05）; as well as telomerase activity was correlated to tumor invasion（P 〈0.001）. [ Conclusions] Telomerase activity and TPS lever may be involved in metastasis of breast cancer, the detection combining telomerase activity with TPS lever has its clinical value in evaluation and treatment of breast cancer.