hTERT mRNA、p53蛋白在胃黏膜病变中的表达及其与幽门螺杆菌感染的关系

Association of expressions of hTERT mRNA and p53 protein in gastric mucosal lesions with Helicobacter pylori infection

目的:探讨人端粒酶催化亚单位(hTERT)mRNA、p53蛋白在胃黏膜病变中的表达和幽门螺杆菌(Hp)感染的关系。方法:对未经抗Hp治疗且被确诊为慢性浅表性胃炎(CSG)和胃癌前病变者,检测胃黏膜Hp感染状况,并分别用RT-PCR和SP免疫组化方法检测hTERT mRNA和p53蛋白的表达。结果:在胃癌前病变组中,hTERT mRNA和p53蛋白的阳性表达率均显著高于CSG组(P<0.05)。在胃癌前病变组中,Hp阳性亚组hTERTmRNA和p53蛋白的表达率分别显著高于Hp阴性亚组的表达率(P<0.05)。在CSG组中,Hp感染和hTERTmRNA、p53蛋白的表达无明显关系。在24例Hp阳性的胃癌前病变组中,p53蛋白和hTERT mRNA的表达有相关性,Pearson相关系数为0.608(P<0.05)。结论:在Hp致胃癌前病变的发生过程中,p53基因的变异和hTERT基因的激活起重要作用,且存在相关关系,这可能是Hp引起胃黏膜病变发生发展的作用机制之一。

Objective To explore the association of expressions of hTERT mRNA and p53 protein in gastric mucosal lesions with Helicobacter pylori （HP） infection. Methods We examined HP infection and detected expressions of hTERT mRNA and p53 protein using RT-PCR and immuhistochemistry in patients with chronic superficial gastritis （CSG） or gastric precancerous lesions （GPL） who had not received anti-HP therapy. Results The expression rates of hTERT mRNA and p53 protein were significantly higher in the GPL group than in the CSG group （P 〈 0.05）. In patients with GPL, the expression rates of hTERT mRNA and p53 protein were markedly higher in HP-positive than in HP-negative （P 〈 0.05）. In the CSG group, HP infection was not associated with the expressions of hTERT mRNA and p53 protein. The expression of hTERT mRNA was associated with that of p53 protein in 24 HP-positve patients with GPL （Pearson＇s corrlation coefficient = 0.608, P 〈 0.05）. Conclusions p53 mutations and hTERT activation, which are associated, play an important role in the genesis of precancerous disease induced by HP infection. They may be one of the mechanisms in the development of gastric mucosal lesions.