RP-HPLC法测定薏苡中薏苡素的含量

Determination of Coixol in Coix lacryma-jobi L. var. mayuen(Roman.) Stapf by RP-HPLC

采用HPLC法，以Diamonsil（R）C18为色谱柱，乙睛-水（50：50）为流动相，流速1mL／min，柱温25℃，检测波长232nm，建立了薏苡非种仁部位中薏苡素的高效液相含量测定方法．结果表明：薏苡素在0．096～1．92μg范围内呈良好的线性关系，该方法的平均回收率为99．12％（n=5），相对标准偏差（RSD）为0．89％，用此条件测得成都华西植物园中薏苡全草中薏苡素的含量为8．3078mg／g．从成分薏苡素和资源综合利用的角度考虑，建议薏苡根、茎和叶可以入药．

Objective To establish an RP-HPLC procedure for determining the content of coixol in the root, stem and leaf of Coix lacryma-jobi L. vat. mayuen （Roman.） Stapf. Methods The Diamonsil（R）C18 column was used with the mobile phase of acetonitrile-water （50： 50）, a flow rate of 1 mL/min, a tempera ture of 25℃ and a wavelength of 232 nm. Results The linear range of coixol was 0. 096-1.92μg. The av erage recovery was 99. 12% （n=5, RSD is 0.89%）. the Botanical Garden of West China was 8. 3078 mg/g. well replicable, and suitable for the determination of C. Coixol in the herbal of C. lacryma-jobi L. var. mayue in Conclusion This method proved to be simple, accurate, lacryma-jobi L. var. mayuen （Roman.） Stapf.