荞麦属(Fagopyrum Mill)植物资源的RAPD研究

Study on RAPD of Genus Fagopyrum Resources

以10个随机引物对荞麦属(Fagopyrum)11个种(含大粒组7个种,小粒组4个种)共50份栽培及野生荞麦资源进行RAPD研究。初步建立了荞麦属不同物种的RAPD指纹图谱。系统聚类分析表明,荞麦属大粒组和小粒组组间以及不同荞麦种间在DNA水平上差异极大。在大粒组中苦荞DNA与其他种之间有较大的差异。大野荞和毛野荞分别与甜荞和苦荞在RAPD水平上较近缘,支持它们分别是甜荞和苦荞祖先种的假说。

50 accessions belonging to 11 Fagopyrum species （ including 7 species in the big-achene-group buckwheat and 4 species in the small-achene-group buckwheat） were studied by means of 10 random primers and Random Amplified Polymorphic DNA （RAPD） technique. The RAPD fingerprint and the phylogeny map of Fagopyrum species were established on the basis of RAPD. Result showed that there were much great differences between the big achene group and the small achene group on DNA level and among different buckwheat species. The differentiation of F. tataricum DNA was the biggest in big achene group, and the differentiation of buckwheat and other species in the big-achene-group was much. F. megaspartanium and F. pilus were very similar to common buckwheat and tartar）, buckwheat, respectively, this phenomenon supported that F. megaspartanium and F. pilus were ancestral species of common buckwheat and tartary buckwheat, respectively.