四乙酰葛根素对离体大鼠胸主动脉环作用初步研究

Study effects of Tetra-acetylated Puerarin on Isolated Rat Thoracic Aortic Rings

目的观察四乙酰葛根素(Tp)对离体大鼠胸主动脉环的作用以及作用机制。方法采用大鼠离体胸主动脉环灌流装置,观测四乙酰葛根素对重酒石酸去甲肾上腺素(NA)和氯化钾(KCl)预收缩主动脉环张力的影响。结果四乙酰葛根素对去甲肾上腺素预收缩主动脉环有明显的舒张作用,与空白对照相比,具有显著性差异(P<0.01);由KCl引起主动脉环预收缩,Tp与空白对照相比,具有统计学差异(P<0.01),对内皮完整血管环的舒张作用明显强于去内皮血管环(P<0.01);Tp对NA在无钙液及正常钙液所致离体血管环收缩的舒张作用不明显(P>0.05)。结论四乙酰葛根素的舒血管作用与受体依赖性钙通道和电压依赖性钙通道均有关,并且其舒血管作用可能与内皮有一定关系。

OBJECTIVE Study effect of tetra-acetylated puerarin on isolated rats thoracic aortic tings and it＇s mechanisms. METHODS The thoracic aortic rings of Sprague-Dawley rats were mounted on a perfusion system, and isometric tensions of the aorta rings precontracted with noradrenaline bitartrate （NA） and potassium chloride （KCl） respectively were recorded in vitro. RESULTS Compared with the controls, tetra-acetylated puerarin showed significantly different vasodilation effects on NA-precontracted aortic ring （P〈0.01） in normal rats; it showed significantly different vasodilation effects on KCl-precontracted aortic ring （P〈0.01）, and show significantly different vasodilation effects on aortic ring with or without endothelium （P〈0.01） in the same molar concentration; it showed no significantly different vasodilation effects on vascular muscle induced calcium-free solution and normal calcium solution （P〉0.05）. CONCLUSION Tetra-acetylated puerarin can inhibit the receptor-operated calcium channel and voltage-dependent calcium channel, and it＇s vasodilator may be related to blood vessel endothelium.