小鼠局部脑损伤对海马区神经元基因表达的影响

The Effects of Local Brain Injury on Bax, Bcl-2 Expression in Mice

目的:探讨局部脑损伤对小鼠海马区Bax,Bcl-2基因表达的影响。方法:40只BALB/c小鼠随机等分成正常组与脑损伤组,用免疫组织化学ABC法检测小鼠海马区Bax,Bcl-2的表达情况。结果:Bax,Bcl-2的免疫性物主要分布于海马区,胞浆染色。小鼠创伤性脑损伤24小时后,神经元Bax,Bcl-2的平均灰度分别为(43.6±3.3)和(54.6±4.2),低于正常组,差异有统计学意义(P<0.05)。结论:脑损伤致海马区Bax,Bcl-2的表达下降。

Objective：To explore the change of Bax,Bcl-2 expression in hippocampus neuron at 24h after local brain Injury.Methods： The 40 mice were divided into normal group and brain injury group,and the expression of Bax and Bcl-2 in hippocampus were determined using ABC immunohistochemical technique.Results： The expression of Bax,Bcl-2 were distributed in hippocampus with stained cytoplasm.Results： At 24h after local brain being injured,the gray degree of Bax,Bcl-2 in neurons of hippocampus were（43.6± 3.3）,（54.6±4.2）,, respectively, which were lower than those in control group （P〈0.01）. Conclusions： The expression of Bax and Bcl-2 were upregulated after local brain being injured.