胆固醇结石患者肝脏LXRα基因的蛋白差异表达及意义

Protein Expression of Liver LXRα Genes in Patients with Cholesterol Gallstone Disease

目的:研究胆囊胆固醇结石患者肝脏的核受体基因:肝脏x受体a(1iver X receptor a,LXRα)、法尼醇受体(farnesoid Xreceptor,FXR)、人类固醇异生物受体(steroid xenobiotic receptor,SXR)及肝受体同类物1(1iver receptor homolog-1,LRH-1)的蛋白表达,探讨胆固醇结石病的发病机理。方法:23例胆囊胆固醇结石患者(胆石组),12例无胆石症的胆囊息肉患者为对照(对照组)。测定胆石胆固醇成分和胆汁脂类成分(胆固醇、磷脂和胆汁酸),并计算胆汁总脂和胆汁胆固醇饱和指数。Western-Blot法测定肝脏LRH-1、FXR、SXR及LXRa基因的蛋白表达量。结果:胆石组胆汁呈胆固醇过饱和;胆汁胆固醇摩尔百分比浓度较对照组升高,P<0.01;胆汁总脂较对照组明显下降,P<0.05;胆汁中胆汁酸和磷脂成分2组比较差异均无统计学意义(P>0.05)。胆石组LRH-1蛋白表达高于对照组(0.88±0.05vs 0.69±0.03),P<0.05,LXRa、FXR和SXR表达2组差异无统计学意义(P>0.05)。

Objective：To investigate the protein expressions of liver X receptorα（LXRa）,farnesoid X receptor（FXR）,steroid xenobiotic receptor（SXR）and liver receptor homolog-1（LRH-1）gene in patients with cholesterol gallstone（CGS）disease,and to elucidate the biomolecular pathogenesis of gallstone formation.Methods： 23 patients with CGS（CGS group） and 12 patients without gallstones（control group）were included in this study.Simultaneously,gallstone cholesterol and biliary composition were assayed.Cholesterol saturation index （CSI） in bile was calculated. Results： CSI was higher in CGS group than that in control group. Biliary cholesterol was also higher in CGS group than that in control group, while biliary total lipid was 1 ower in CGS group than that in control group. There were no differences in bile salts and lecithin between two groups （P〉0.05）.Protein expression of LRH-I gene was higher in CGS group than that in control group （0.88± 0.05vs 0.69± 0.03）, the difference was statistically significant （P〈0.05）. There were no differences in protein expressions ofLXRa, FXR and SXR gene between two groups （P〉0.05）. Conclusion： CGS disease may be related to increased protein expression of LRH-1 gene.