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DLK1基因在急性白血病细胞系中的表达及其意义 被引量：1

Expression and significance of DLK1 gene in acute leukemia cell lines
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摘要 目的:研究急性白血病细胞系DLK1基因的表达水平在红系分化中的作用。方法:采用RT-PCR、Western bltting对白血病细胞系K562、HL-60进行DLK1水平的检测。培养K562细胞,用氯化高铁血红素(hemin)诱导其分化,观察DLK1在红系分化中的变化。结果:K562细胞DLK1mRNA、蛋白水平存在明显表达,HL-60细胞DLK1则不表达。通过RT-PCR检测了hemin诱导K562细胞向红系分化过程中各时间点DLK1mRNA的变化,显示随着K562向红系分化,DLK1mRNA的水平逐渐下降。结论:K562细胞表达DLK1,HL-60不表达DLK1。DLK1基因可能参与K562细胞向红系分化的过程,可能抑制其分化。 Objectives： To investigate the expression of DLK1 gene in leukemia cell lines and the function during erythroid differentiation. Methods： To detect the expression of DLK1 gene in leukeamia cell lines such as K562 and HL-60 with RT-PCR and western blotting. The K562 cell line was induced to erythroid differentiation by hemin, then observe the relationship between it＇s expression and erythroid differentiation. Results： MRNA and protein of DLK1 was expressed in K562 cells while not expressed in HL-60 cells. To explore changes of DLK1 gene hemin-induced expression at each time point during erythroid differentiation, indicating that DLK1 mRNA decreased gradually along with K562 cells towards erythroid differentiation. Conclusions： DLK1 was expressed in K562 cells while not expressed in HL-60 cells. DLK1 is possibly involved in the process of erythroid differen- tiation ofK562 cells, the present study shows it may inhibit the process.
作者 龙潺 唐雪元 许诚
机构地区 深圳市第三人民医院 中南大学湘雅三医院
出处 《现代生物医学进展》 CAS 2009年第19期3713-3715,F0003,共4页 Progress in Modern Biomedicine
关键词 急性白血病细胞系 DLK1 基因表达 红系分化 Acute leukemia cell lines DLK1 Gene expression Erythroiddifferentiation
分类号 R733.71 [医药卫生—肿瘤]
  • 相关文献

参考文献7

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共引文献5

同被引文献26

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现代生物医学进展
2009年 第19期

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