上海地区汉族人群LMTK2与MSMB基因多态性与前列腺癌的易感性

LMTK2 and MSMB gene polymorphisms and genetic susceptibility to prostate cancer in the Han population in Shanghai

目的:探讨中国上海地区汉族人群中LMTK2与MSMB基因多态性与前列腺癌遗传易感性的关系。方法:采用病例对照研究,提取200例前列腺癌患者(病例组)和200例非前列腺癌健康人(对照组)外周血中基因组DNA,应用ABI3730XL测序仪分析病例组和对照组的LMTK2基因rs6465657位点以及MSMB基因rs10993994位点的多态性,比较不同基因型与前列腺癌易感性的关系。结果:MSMB基因rs10993994位点密码子T/C基因型的个体其前列腺癌发病风险是C/C基因型的1.62倍(OR=1.62,95%可信区间:1.12～2.27),携带MSMB基因rs10993994位点等位基因T(T/T,T/C)的个体发生前列腺癌的风险性是C/C基因型的0.96倍(OR=0.96,95%可信区间:0.82～1.11)。LMTK2基因rs6465657位点密码子C/C基因型的个体其前列腺癌发病风险与T/C基因型无明显差异。结论:中国上海地区汉族人群中MSMB基因rs10993994位点多态性可能对前列腺癌遗传易感性有影响,而LMTK2基因rs6465657位点对前列腺癌遗传易感性无明显影响。

OBJECTIVE： To investigate the correlation between LMTK2, MSMB gene polymorphisms and genetic susceptibility to prostate cancer in the Han population in Shanghai. METHODS： A case control experiment was performed, consisting of 200 cases with newly diagnosed prostate cancer and 200 cancer-free healthy controls. Then peripheral blood genome DNA was extracted. ABI 3730 XL sequenator was used to determine the polymorphism of LMTK2 gene at rs6465657 site and MSMB gene at rs10993994 site. The correlation between the two gene and susceptibility to prostate cancer. RESULTS： MSMB gene at rs10993994 site genetype TIC had a large risk to get prostate cancer compared with genetype C/C （OR=1.62, 95% CI 1.12-2.27）. MSMB gene at rs10993994 site allele T （T/T, T/C） had a large risk to get prostate cancer compared with genetype C/C （OR=0.96, 95% CI 0.82-1.11）. LMTK2 gene at rs6465657 site did not have significant difference between C/C genetype and TIC genetype. CONCLUSION： MSMB gene at rs10993994 site polymorphism may influence the genetic susceptibility to prostate cancer in the Han population in Shanghai, while LMTK2 gene at rs6465657 site has no obvious effect.