特拉唑嗪诱导前列腺癌PC-3细胞凋亡的实验研究

Effects of terazosin on cell proliferation and apoptosis in PC-3 cell line.

目的探讨α1肾上腺素受体拮抗剂特拉唑嗪对激素非依赖性前列腺癌细胞株PC-3细胞凋亡的影响。方法应用不同浓度特拉唑嗪处理对数生长期的PC-3细胞,以MTT比色法检测细胞生长抑制率,流式细胞术(FCM)检测细胞凋亡和细胞周期变化,Western-Blot检测凋亡相关蛋白Caspase-7和PARP表达的变化。结果应用15μmol/L、25μmol/L、50μmol/L特拉唑嗪分别处理PC-3细胞24 h及48 h后,随着药物浓度的增加和处理时间的延长,细胞生长受到抑制,凋亡细胞增多,部分细胞形态学结构破坏。Western-Blot法检测显示PC-3细胞凋亡的增加与PARP的降解有关,与Caspase-7的表达无明显关系。结论特拉唑嗪对PC-3细胞的生长抑制呈剂量依赖关系,并可能通过Caspase-PARP凋亡通路诱导PC-3细胞凋亡。

Objective To investigate the biological efficiency of terazosin on human prostate cancer PC - 3 cells. Methods Exponentially growing PC3 cells were exposed to terazosin with 15 μmol/L,25 μmoL/L and 50 μmol/L for 24 h and 48h respectively. The cell growth rate, apoptotic rate and cell cycle were measured by MTT and flow cytometry, and the expression of caspase - 7 and PARP were analyzed by Western - Blot. Results With the increasing concentration of terazosin and the incubation time, cell growth was inhibited, and number apoptotic cells increased. The apoptosis of PC - 3 cells were correlated with PARP degradation, but not with caspase - 7 according to Western - Blot analysis. Conclusion There is a positive relation between the cell growth inhibition, cytotoxic activity and the dose of terazosin, and the mechanism of terazosin - induced cell apoptosis in PC - 3 cells may be through the Caspase -PARP pathway.