摘要
利用非损伤取样方法,从湖北石首麋鹿的粪便样品中提取得到其线粒体基因组Cyt b基因的部分序列,并对其进行了系统发育分析.结果表明:现有的麋鹿是遗传多样性高度特化的物种;在分歧时间上,麋鹿属较黇鹿属更晚从鹿亚科中分化独立出来,与鹿属中的坡鹿有着较近的进化关系.此外,还对有关糜鹿归属的古生物学和分子系统学研究结果出现矛盾的原因进行了初步分析,认为通过对我国多地点不同年代地层中麋鹿化石或亚化石进行古DNA研究,可弥补现有分子系统学研究中由于奠基者效应带来的取样误差,得到更有说服力的分析结果.
Partial DNA sequence of cytochrome b gene in mitochondrial genome was successfully retrieved from faeces of Pere David＇s deer （Elaphurus davidianus） collected from Shishou, Hubei Province in central China. Phylogenetic analysis using the sequence and published data indicated that the genetic diversity in Pete David＇s deer is quite limited; Elaphurus has diverged from Cervinae later than the genera Dama and has a close phylogenetic relationship to Cervus eldi. We suggest that the molecular system- atics efforts is essential to be continued and the ancient DNA study based on fossils and sub-fossils in the temporal and the spatial ranges might be an efficient way to solve the sampling problems, which caused by founder effect in Pere David＇s deer.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第3期468-473,共6页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金资助项目(40672006)
生物地质与环境地质教育部重点实验室(中国地质大学)开放基金资助项目(BGEG0604)
关键词
分子系统学
麋鹿
线粒体DNA
系统发育分析
molecular systematics
Elaphurus davidianus
mitochondrial DNA
phylogenic analysis
