磷脂酶Dβ在拟南芥低温信号中的转导作用

The Role of Phospholipase Dβ on Signal Transduction of Low Temperature in Arabidopsis thaliana

磷脂酶D(PLD)不仅是植物中一类主要的磷脂水解酶,而且是一类重要的跨膜信号转导酶类。PLD的磷脂降解功能和信号转导功能均影响植物的抗冻性。本研究以PLDβ基因被敲除的拟南芥突变体及其野生型植株为材料,进行低温驯化和冻害胁迫处理,并分析其作用途径。结果表明,PLDβ基因介导低温信号转导作用,参与渗透调节途径中脯氨酸的调控和抗氧化系统中过氧化氢酶(CAT)活性的调控,并且与低温信号激素ABA不在同一条信号转导途径。本研究为探索通过调控PLD的活性提高植物抗冻性提供了新的途径,并为深入揭示植物的抗冻机理以及磷脂信号转导机制提供实验支持。

PhospholipaseD（PLD） isnotonly a major kind ofphospholipid hydrolase in plant, butalsoa mainkindof trans-membrane transduction enzyme. Both of the phospholipid degradation function and signal transduction function could affect plant freezing tolerance. In this research, we employed the A rabidopsis thaliana mutants in which PLDfl gene was knocked out and the wild type strain as the tested materials by treatment with low temperature acclimated and freezing stressed and analyze their function. The results showed that the PLDfl gene mediated signal transduction function of low temperature as well as involved in regulating proline level during the process of osmotic adjustment and regulating CAT activity in the anti-oxidation system. Further results illuminated that their signal transduction pathway was not in the same one with the pathway of low temperature signal hormone ABA. This study would provide a new approach for exploring how to improve the plant freezing tolerance by regulating the activity of PLD, and also could deeply reveal the mechanism of plant freezing tolerance and also will give some experimental support for the mechanism ofphospholipid signal transduction.