不结球白菜新种质P70-203雄性不育细胞质类型的鉴定

Identification of Cytoplasmic Male Sterile Type of P70-203, a New Germplasm of Non-heading Chinese Cabbage

本研究根据OguraCMS、PolimaCMS的不育性状相关的线粒体基因序列设计特异引物,对不结球白菜雄性不育系新种质P70-203及其保持系P60-27-1进行PCR分析。研究结果表明,Polima引物P3/P4,P5/P6在不育系与可育系中均无扩增条带;Ogura引物P1/P2在不育系中扩增出750bp的特异片段,但可育系中无扩增条带。将扩增的特异条带回收并测序,将得到的测序结果在NCBI中进行Blastn同源性比较,结果与青花菜Ogura(登录号:EU604643)和萝卜Ogura(登录号:AB055438)细胞质雄性不育同源性均达到99%。从分子角度初步推测:该雄性不育系新种质P70-203具有Ogura细胞质。

In this research, we analyzed the type of cytoplasmic male sterile line of non-heading Chinese cabbage, P70-203, and its maintainer, P60-27-1, by PCR approaches based on the specific primer pairs designed from the mitochondrial genes of PolimaCMS and the OguraCMS, which would be close related to the cytoplasmic male sterile characters. The results showed that the specific 750 bp length of fragments from the tested male sterile line was amplified by using Ogura primer pair P1/P2, nor from the maintaining lines as well. And also the primers of Polima P3/P4, P5/P6 was in available for amplifying in the both of the tested male sterile line and its maintainer. Blastn analysis NCBI online indicated that the amplified 750 bp length DNA shares 99% high homology with that of the mitochondrial gene of Ogura cytoplasmic male sterile line in broccoli （Accession number： EU604643）, and the same degree of homology are found between the 750 bp segment and the mitochondrial gene of the Ogura cytoplasmic male sterile line in radish （Accession number. AB055438）. These results could reveal that the cytoplasmic male sterile of P70-203, a New germplasm of non-heading Chinese cabbage, might belong to the type of Ogura cytoplasmic sterile.