南通地区乙型肝炎病毒基因分型及其临床意义研究

Investigation of hepatitis B virus genotype and the clinical manifestation in Nantong city

目的了解南通地区乙型肝炎病毒基因分型情况及其临床意义。方法用PCR法扩增HBV PreS基因并测序,共分析慢性无症状携带者(ASC)、慢性活动性肝炎患者(CAH)和肝癌(HCC)患者血清86份。用C lustalx1.8软件比对,用MEGA3.1作树分型,同时测定样品HBeAg和HBV-DNA。结果南通地区存在HBV B型、C型和B/C混合感染型,其中C型比例最高,占67.44%,而B型和B/C混合感染型均占16.28%。C型患者的血清HBeAg阳性率和HBV-DNA浓度显著高于其他基因型(p<0.05)。结论南通地区存在HBV B型、C型以及B+C混合型,C型为优势型;C型患者的病毒复制最为活跃。

Objective To study the distribution of hepatitis B virus in Nantong city and the clinical implications. Methods The gene of HBV PreS from HBV -DNA patients including asymptomatic HBV carriers （ASC）, chronic active hepatitis I CAH） and hepatocarcinoma （ HCC） were amplified with PCR and sequenced. The sequences of all samples were analyzed using Clustalxl. 8 and MEGA3.1. Serum HBeAg and HBV - DNA were assayed for the marker of virus replication. Results Of the 86 samples, 67.44 % were genotype C and genotype B and the B/C mix - infected were both 16.28 %. The clinical manifestations demonstrated that serum HBeAg and HBV - DNA concentration in patients of genotype C were higher than that of other groups. Conclusion C, enotype C was the dominating genotype in Nantong city in all HBV infections. Replication of HBV is the most active in genotype C patients.