摘要
目的建立加味八珍益母颗粒中盐酸水苏碱含量的测定方法。方法采用HPLC法,强酸型阳离子交换色谱柱,流动相为0.05mol.L-1磷酸二氢钾溶液-三乙胺（100∶0.1）（磷酸调节pH值2.6）,检测波长为190nm。结果盐酸水苏碱在0.8384~8.384μg内,峰面积与进样量呈良好的线性关系,r=0.9999（n=6）;平均回收率：98.2%,RSD=1.37%（n=6）。结论测定方法准确、重现性好,为加味八珍益母颗粒的质量控制提供了依据。
Objective To establish a method to determination of stachydrine hydrochloride in Bazhen Yimu Granules.Methods The samples were determined by HPLC on a strong acidic cation exchange resin column with 0.05mol·L-1 solution of potassium dihydrogen phosphate-trithylamine（100∶0.1）（adjust to pH 2.6 with phosphoric acid） as the mobile phase.Detective wave length was at 190nm.Results The content of stachydrine hydrochloride on HPLC was linear in the range of 0.8384~8.384μg（r=0.9999,n=6）.The average recovery was 98.2%, and RSD was 1.37% （n=6）. Conclusions The method is accurate and repeatable. The method can be used for control of Bazhen Yimu Granules.
出处
《中国药事》
CAS
2009年第11期1113-1114,1122,共3页
Chinese Pharmaceutical Affairs
