摘要
以中苎一号叶片为外植体,研究了外植体消毒时间、培养基、生长调节物质对苎麻品种中苎一号叶片愈伤诱导、分化不定芽和生根的影响。其结果表明:先用75%酒精消毒10s,再用0.1%升汞灭菌8min,同时滴入1-2滴l%HCI效果最好。中苎一号最佳叶片愈伤组织诱导基本培养基为1/2MS;最佳叶片愈伤组织诱导培养基组合为:1/2Ms+0.05mg/LTDZ+o.01mg/LIAA+0.03mg/L2,4.D;最佳愈伤分化培养基为:1/2MS+0.5mg/LTDZ+0.02mg/L2,4-D+0.03mg/LIAA;最佳生根培养基为:l/2MS+0.02mg/LTDZ+0.05mg/LNAA+0.02mg/LIAA,形成了较为完善的组织培养再生体系,再生率达到了26%以上。
Based on the duration of disinfection, leaf-blades of Ramie being for the outside plants body, this paper researches on the culture medium and the growth of adjustment materials which affect the leaves of Ra- mie's callus induction, the occurrence of adventitious buds and rooting. The result shows that, firstly, using 75% of alcohol to have disinfection in 10 seconds; then, using 0.1% mercuric chloride to have sterilization in 8 minutes, dripping into 1-2 drop of 1% HC1 at the same time, it is the optimum way to cause effective result. The best leaf of Ramiels callus induction basic culture medium is 1/2MS. The best leaves induction organiza- tion culture medium combination is that: 1/2MS+0.05 mg/L TDZ+0.01 mg/L IAA+0.03 mg/L 2,4-D; The opti- mum injury differentiation medium is that: 1/2MS+0.5 mg/L TDZ+0.02 mg/L 2,4-D+0.03 mg/L IAA; The best rooting medium is that: 1/2MS+0.02 mg/L TDZ+O.05 mg/L NAA+0.02 mg/L IAA. Thus, it has formed the more perfect tissue culture regeneration system, regeneration rate has reached 26% the above.
出处
《中国农学通报》
CSCD
北大核心
2009年第23期86-89,共4页
Chinese Agricultural Science Bulletin
基金
湖南农业大学人才引进科技资助项目"苎麻优异基因鉴定
克隆与表达分析"(07YT03)
关键词
苎麻
叶片
组织培养
植株
再生
Ramie, leaf, tissue culture, plant, regenerate