苎麻叶片组织培养再生植株的研究

Study on Regeneration Plants by Tissue Culture Used Leaf Explant in Ramie

以中苎一号叶片为外植体，研究了外植体消毒时间、培养基、生长调节物质对苎麻品种中苎一号叶片愈伤诱导、分化不定芽和生根的影响。其结果表明：先用75％酒精消毒10s，再用0．1％升汞灭菌8min，同时滴入1-2滴l％HCI效果最好。中苎一号最佳叶片愈伤组织诱导基本培养基为1／2MS；最佳叶片愈伤组织诱导培养基组合为：1／2Ms＋0．05mg／LTDZ＋o．01mg／LIAA＋0．03mg／L2，4．D；最佳愈伤分化培养基为：1／2MS＋0．5mg／LTDZ＋0．02mg／L2，4-D＋0．03mg／LIAA；最佳生根培养基为：l／2MS＋0．02mg／LTDZ＋0．05mg／LNAA＋0．02mg／LIAA，形成了较为完善的组织培养再生体系，再生率达到了26％以上。

Based on the duration of disinfection, leaf-blades of Ramie being for the outside plants body, this paper researches on the culture medium and the growth of adjustment materials which affect the leaves of Ra- mie＇s callus induction, the occurrence of adventitious buds and rooting. The result shows that, firstly, using 75% of alcohol to have disinfection in 10 seconds; then, using 0.1% mercuric chloride to have sterilization in 8 minutes, dripping into 1-2 drop of 1% HC1 at the same time, it is the optimum way to cause effective result. The best leaf of Ramiels callus induction basic culture medium is 1/2MS. The best leaves induction organiza- tion culture medium combination is that： 1/2MS＋0.05 mg/L TDZ＋0.01 mg/L IAA＋0.03 mg/L 2,4-D; The opti- mum injury differentiation medium is that： 1/2MS＋0.5 mg/L TDZ＋0.02 mg/L 2,4-D＋0.03 mg/L IAA; The best rooting medium is that： 1/2MS＋0.02 mg/L TDZ＋O.05 mg/L NAA＋0.02 mg/L IAA. Thus, it has formed the more perfect tissue culture regeneration system, regeneration rate has reached 26% the above.