He-Ne激光对增强UV-B辐射后小麦幼苗多胺及相关酶活性的影响

On Polyamine Contents and Polyamine Oxidase Activity of Leaves in Wheat Seedings Exposed to Enhanced Ultraviolet-B Radiation and irradiated by He-Ne laser

采用He-Ne激光辐照对增强UV-B辐射后小麦幼苗的损伤修复作用进行了研究。小麦种子在盛有湿滤纸的培养皿内在25℃下进行萌发。萌发后小麦幼苗再经10.08 kJ(/m2.天)的增强UV-B辐射,然后再用10 mW/mm2的He-Ne激光进行辐照。通过小麦幼苗叶片多胺含量、多胺氧化酶活性及膜脂过氧化物的变化,测定了He-Ne激光对小麦UV-B损伤的修复情况。结果表明,多胺、多胺氧化酶、膜脂过氧化物的变化同小麦幼苗损伤的修复的能力相关联。He-Ne激光辐照可使由增强UV-B辐射后诱导叶片升高的多胺含量降低。增强UV-B辐射对多胺氧化酶活性和膜脂过氧化物呈促进的作用。显示He-Ne激光对多胺氧化酶由于增强UV-B辐照造成的伤害有一定的修复。

The seedlings of wheat were exposed to He-Ne laser irradiation after enhanced UV-B radiated, in order to determine some physiological traits in the damage induced by UV-B radiation. The seeds were culti- vated in Petridishes in a constant temperature incubator （25 ℃） until they were germinated. The germinated seeds were exposed to 10.08 kJ/（/m2· d） UV-B radiations, one by one, and then they were irradiated by He-Ne laser with 10 mW/mm2 Power density. Changes about the polyamine contents （PAs） and the activity of the poly- amine oxidase（PAO） and the content of MDA were measured to test the repair role of laser irradiation. The re- suits showed that the damage of wheat seedlings induced by enhanced UV-B radiation in physiology can be re- paired partly by He-Ne laser irradiation with decreasing the content of PAs and raising the activity of PAO. This is the first investigation reporting the use of laser irradiation treatment to repair the wheat damage induced by UV-B radiation in peroxidase and polyamine oxidaset.